Peripheral neuronal activation shapes the microbiome and alters gut physiology/Experiment 4
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-4-30
Curated date: 2025/04/17
Curator: PreciousChijioke
Revision editor(s): PreciousChijioke, KateRasheed
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Cecum mucosa Caecum mucosa,Caecum mucosa of organ,Caecum mucous membrane,Caecum organ mucosa,Cecal mucosa,Cecum mucosa of organ,Cecum mucous membrane,Cecum organ mucosa,Intestinum crassum caecum mucosa,Intestinum crassum caecum mucosa of organ,Intestinum crassum caecum mucous membrane,Intestinum crassum caecum organ mucosa,Mucosa of caecum,Mucosa of cecum,Mucosa of intestinum crassum caecum,Mucosa of organ of caecum,Mucosa of organ of cecum,Mucosa of organ of intestinum crassum caecum,Mucous membrane of caecum,Mucous membrane of cecum,Mucous membrane of intestinum crassum caecum,Organ mucosa of caecum,Organ mucosa of cecum,Organ mucosa of intestinum crassum caecum,Cecum mucosa,cecum mucosa
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Peripheral neuron Peripheral neuron,peripheral neuron
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control mice on day 10
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- TH+ (Tyrosine hydroxylase) -activated mice on day 10
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- TH+ (Tyrosine hydroxylase) -activated mice were treated with C21 ( compound 21) (3 mg/kg) administered daily for 10 consecutive days.
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-4-30
Source: Fig S6
Description: Annotated changes in gut bacteria
Abundance in Group 1: increased abundance in TH+ (Tyrosine hydroxylase) -activated mice on day 10
Revision editor(s): PreciousChijioke
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-4-30
Source: Fig S6
Description: Annotated changes in gut bacteria
Abundance in Group 1: decreased abundance in TH+ (Tyrosine hydroxylase) -activated mice on day 10
Revision editor(s): PreciousChijioke
