The oral cavity and intestinal microbiome in children with functional constipation/Experiment 1

From BugSigDB


Needs review

Curated date: 2025/03/12

Curator: Tosin

Revision editor(s): Tosin

Subjects

Location of subjects
Poland
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Chronic constipation Chronic constipation,Infrequent bowel movements,chronic constipation
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Children without constipation
Group 1 name Corresponds to the case (exposed) group for case-control studies
Children with constipation
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Children over three years of age with constipation
Group 0 sample size Number of subjects in the control (unexposed) group
34
Group 1 sample size Number of subjects in the case (exposed) group
57
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Kruskall-Wallis
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Pielou Quantifies how equal the community is numerically
increased
Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
increased

Signature 1

Needs review

Curated date: 2025/03/14

Curator: Tosin

Revision editor(s): Tosin

Source: Table 3

Description: Taxonomic changes in the gut microbiome in children with functional constipation (FC) compared to children without functional constipation (FC)

Abundance in Group 1: increased abundance in Children with constipation

NCBI Quality ControlLinks
Tannerellaceae

Revision editor(s): Tosin

Signature 2

Needs review

Curated date: 2025/03/14

Curator: Tosin

Revision editor(s): Tosin

Source: Table 3

Description: Taxonomic changes in the gut microbiome in children with functional constipation (FC) compared to children without functional constipation (FC).

Abundance in Group 1: decreased abundance in Children with constipation

NCBI Quality ControlLinks
Burkholderiaceae
Christensenellaceae
Clostridiaceae
Fusicatenibacter
Parabacteroides
Romboutsia
Subdoligranulum

Revision editor(s): Tosin