Effect of a high-fat high-fructose diet on the composition of the intestinal microbiota and its association with metabolic and anthropometric parameters in a letrozole-induced mouse model of polycystic ovary syndrome
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Pieczyńska-Zając JM, Malinowska AM, Pruszyńska-Oszmałek E, Kołodziejski PA, Drzymała-Czyż S, Bajerska J
Journal
Nutrition (Burbank, Los Angeles County, Calif.)
Year
2024
Keywords:
Intestinal microbiota, Lipopolysaccharide, Reproductive disorders, Short-chain fatty acids
OBJECTIVE: It has been suggested that dysbiosis of the gut microbiota is associated with the pathogenesis of Polycystic Ovary Syndrome (PCOS), and that improper diet can aggravate these changes. This study thus aimed to investigate the effects of a high-fat/high-fructose (HF/HFr) diet on the gut microbial community and their metabolites in prepubertal female mice with letrozole (LET)-induced PCOS. We also tested the correlations between the relative abundance of microbial taxa and selected PCOS parameters. RESEARCH METHODS & PROCEDURES: Thirty-two C57BL/6 mice were randomly divided into four groups (n = 8) and implanted with LET or a placebo, with simultaneous administration of a HF/HFr diet or standard diet (StD) for 5 wk. The blood and intestinal contents were collected after the sacrifice. RESULTS: Placebo + HF/HFr and LET + HF/HFr had significantly higher microbial alpha diversity than either group fed StD. The LET-implanted mice fed StD had a significantly higher abundance of Prevotellaceae_UCG-001 than the placebo mice fed StD. Both groups fed the HF/HFr diet had significantly lower fecal levels of short-chain fatty acids than the placebo mice fed StD, while the LET + HF/HFr animals had significantly higher concentrations of lipopolysaccharides in blood serum than either the placebo or LET mice fed StD. Opposite correlations were observed between Turicibacter and Lactobacillus and the lipid profile, CONCLUSION: HF/HFr diet had a much stronger effect on the composition of the intestinal microbiota of prepubertal mice than LET itself.
Experiment 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Subjects
- Location of subjects
- Poland
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Caecum Blind intestine,Blindgut,Caeca,Caecum,Ceca,Cecum,Intestinum caecum,Intestinum crassum caecum,Intestinum crassum cecum,caecum
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to diet Response to diet,response to diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Placebo
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Placebo+high-fat/high-fructose (HF/HFr) diet
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice in this group were injected with a placebo pellet and fed a high-fat/high-fructose diet.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Dunn's test
- Kruskall-Wallis
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Source: Table 1 & Supplementary Table S1
Description: The relative abundances of selected taxa at all taxonomic levels in intestinal microbiota of prepubertal female C57BL/6 mice.
Abundance in Group 1: increased abundance in Placebo+high-fat/high-fructose (HF/HFr) diet
Revision editor(s): Victoria
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Source: Table 1 & Supplementary Table S1
Description: The relative abundances of selected taxa at all taxonomic levels in intestinal microbiota of prepubertal female C57BL/6 mice.
Abundance in Group 1: decreased abundance in Placebo+high-fat/high-fructose (HF/HFr) diet
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillales | ||
| Lactobacillus | ||
| Lactobacillaceae |
Revision editor(s): Victoria
Experiment 2
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Polycystic ovary syndrome Cystic disease of ovaries,hyperandrogenemia,Multicystic ovaries,multicystic ovaries,Ovarian Degeneration, Sclerocystic,Ovarian Syndrome, Polycystic,Ovarian Syndromes, Polycystic,Ovaries, Sclerocystic,Ovary Syndrome, Polycystic,Ovary, Sclerocystic,PCO - Polycystic ovaries,Pco1,PCOD - Polycystic ovarian disease,PCOS,Pcos,PCOS - Polycystic ovarian syndrome,PCOS1,Polycystic ovarian disease,polycystic ovarian disease,Polycystic ovarian syndrome,Polycystic ovaries,polycystic ovaries,Polycystic ovaries (disorder),polycystic ovary,polycystic ovary syndrome,polycystic ovary syndrome 1,Sclerocystic Ovarian Degeneration,Sclerocystic Ovaries,Sclerocystic Ovary,Sclerocystic Ovary Syndrome,Stein Leventhal Syndrome,Stein-Leventhal synd.,Stein-Leventhal Syndrome,Stein-Leventhal syndrome,Syndrome, Polycystic Ovary,Syndrome, Stein-Leventhal,Polycystic ovary syndrome
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- letrozole-induced polycystic ovary syndrome (LET)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice in this group were injected with a Letrozole (LET) pellet to induce polycystic ovary syndrome (PCOS) and fed a standard diet.
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Source: Table 1 & Supplementary Table S1
Description: The relative abundances of selected taxa at all taxonomic levels in intestinal microbiota of prepubertal female C57BL/6 mice.
Abundance in Group 1: increased abundance in letrozole-induced polycystic ovary syndrome (LET)
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotellaceae_UCG-001Prevotellaceae_UCG-001 |
Revision editor(s): Victoria
Experiment 3
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to diet Response to diet,response to diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Letrozole-induced polycystic ovary syndrome (LET)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Placebo+high-fat/high-fructose (HF/HFr) diet
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice in this group were injected with a placebo pellet and fed a high-fat/high-fructose diet.
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Source: Table 1 & Supplementary Table S1
Description: The relative abundances of selected taxa at all taxonomic levels in intestinal microbiota of prepubertal female C57BL/6 mice.
Abundance in Group 1: increased abundance in Placebo+high-fat/high-fructose (HF/HFr) diet
Revision editor(s): Victoria
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Source: Table 1 & Supplementary Table S1
Description: The relative abundances of selected taxa at all taxonomic levels in intestinal microbiota of prepubertal female C57BL/6 mice.
Abundance in Group 1: decreased abundance in Placebo+high-fat/high-fructose (HF/HFr) diet
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotellaceae NK3B31 groupPrevotellaceae NK3B31 group | ||
| Lactobacillus | ||
| Butyricicoccus | ||
| Lactobacillaceae | ||
| Lactobacillales |
Revision editor(s): Victoria
Experiment 4
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Letrozole-induced polycystic ovary syndrome (LET)+high-fat/high-fructose (HF/HFr) diet
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Source: Table 1 & Supplementary Table S1
Description: The relative abundances of selected taxa at all taxonomic levels in intestinal microbiota of prepubertal female C57BL/6 mice.
Abundance in Group 1: increased abundance in Placebo+high-fat/high-fructose (HF/HFr) diet
Revision editor(s): Victoria
Experiment 5
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Letrozole-induced polycystic ovary syndrome (LET)+high-fat/high-fructose (HF/HFr) & Placebo+high-fat/high-fructose (HF/HFr) & Letrozole-induced polycystic ovary syndrome (LET)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Placebo
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice in this group were injected with a placebo pellet and fed a standard diet.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 24
Lab analysis
Statistical Analysis
- Statistical test
- LEfSe
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Source: Supplementary Figure S1 & S2
Description: Cladogram of LEfSe analysis of gut microbiota in different groups. The microbial compositions were compared at five taxonomic levels (phylum, class, order, family, genus).
Abundance in Group 1: increased abundance in Placebo
| NCBI | Quality Control | Links |
|---|---|---|
| Lachnospirales | ||
| Deferribacteres | ||
| Deferribacterota | ||
| Deferribacterales | ||
| Deferribacteraceae | ||
| Mucispirillum | ||
| Alistipes | ||
| Lachnospiraceae | ||
| Lactobacillales | ||
| Lactobacillaceae | ||
| Lactobacillus |
Revision editor(s): Victoria
Experiment 6
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Polycystic ovary syndrome Cystic disease of ovaries,hyperandrogenemia,Multicystic ovaries,multicystic ovaries,Ovarian Degeneration, Sclerocystic,Ovarian Syndrome, Polycystic,Ovarian Syndromes, Polycystic,Ovaries, Sclerocystic,Ovary Syndrome, Polycystic,Ovary, Sclerocystic,PCO - Polycystic ovaries,Pco1,PCOD - Polycystic ovarian disease,PCOS,Pcos,PCOS - Polycystic ovarian syndrome,PCOS1,Polycystic ovarian disease,polycystic ovarian disease,Polycystic ovarian syndrome,Polycystic ovaries,polycystic ovaries,Polycystic ovaries (disorder),polycystic ovary,polycystic ovary syndrome,polycystic ovary syndrome 1,Sclerocystic Ovarian Degeneration,Sclerocystic Ovaries,Sclerocystic Ovary,Sclerocystic Ovary Syndrome,Stein Leventhal Syndrome,Stein-Leventhal synd.,Stein-Leventhal Syndrome,Stein-Leventhal syndrome,Syndrome, Polycystic Ovary,Syndrome, Stein-Leventhal,Polycystic ovary syndrome
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Letrozole-induced polycystic ovary syndrome (LET)+high-fat/high-fructose (HF/HFr) & Placebo+high-fat/high-fructose (HF/HFr) & Placebo
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Letrozole-induced polycystic ovary syndrome (LET)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice in this group were injected with a Letrozole (LET) pellet to induce polycystic ovary syndrome (PCOS) and fed a standard diet.
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Source: Supplementary Figure S1 & S2
Description: Cladogram of LEfSe analysis of gut microbiota in different groups. The microbial compositions were compared at five taxonomic levels (phylum, class, order, family, genus).
Abundance in Group 1: increased abundance in Letrozole-induced polycystic ovary syndrome (LET)
| NCBI | Quality Control | Links |
|---|---|---|
| Butyricicoccus | ||
| Prevotellaceae NK3B31Prevotellaceae NK3B31 | ||
| Prevotellaceae UCG-001Prevotellaceae UCG-001 |
Revision editor(s): Victoria
Experiment 7
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to diet Response to diet,response to diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Letrozole-induced polycystic ovary syndrome (LET)+high-fat/high-fructose (HF/HFr) & Placebo & Letrozole-induced polycystic ovary syndrome (LET)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Placebo+high-fat/high-fructose (HF/HFr)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice in this group were injected with a placebo pellet and fed a high-fat/high-fructose diet.
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Source: Supplementary Figure S1 & S2
Description: Cladogram of LEfSe analysis of gut microbiota in different groups. The microbial compositions were compared at five taxonomic levels (phylum, class, order, family, genus).
Abundance in Group 1: increased abundance in Placebo+high-fat/high-fructose (HF/HFr)
Revision editor(s): Victoria
Experiment 8
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Placebo+high-fat/high-fructose (HF/HFr) & Placebo & Letrozole-induced polycystic ovary syndrome (LET)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Letrozole-induced polycystic ovary syndrome (LET)+high-fat/high-fructose (HF/HFr)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice in this group were injected with a Letrozole pellet and fed a high-fat/high-fructose diet.
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-11
Source: Supplementary Figure S1 & S2
Description: Cladogram of LEfSe analysis of gut microbiota in different groups. The microbial compositions were compared at five taxonomic levels (phylum, class, order, family, genus).
Abundance in Group 1: increased abundance in Letrozole-induced polycystic ovary syndrome (LET)+high-fat/high-fructose (HF/HFr)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidales | ||
| Bacteroidota | ||
| Bacteroidia | ||
| Muribaculaceae | ||
| Muribaculum | ||
| Bilophila | ||
| TuzzerellaTuzzerella |
Revision editor(s): Victoria
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