Microbial imbalance in Chinese children with diarrhea or constipation/Experiment 6

From BugSigDB


Reviewed Marked as Reviewed by KateRasheed on 2025-5-2

Curated date: 2025/04/30

Curator: Ameenatoloko

Revision editor(s): Ameenatoloko

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Diarrhea Diarrhea,Diarrhoea,Watery stool,diarrhea
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy Controls (HC)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Children with diarrhea (CD)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Children with diarrhea experience a range of health impacts, such as difficulty in feeding, reduced immunity, malnutrition, and foodborne illness, with potential complications like viral myocarditis.
Group 0 sample size Number of subjects in the control (unexposed) group
398
Group 1 sample size Number of subjects in the case (exposed) group
60
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
decreased
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-5-2

Curated date: 2025/04/30

Curator: Ameenatoloko

Revision editor(s): Ameenatoloko

Source: Fig. S4, Table S8

Description: A cladogram made by LEfSe demonstrates different bacterial taxa between the CD and HC groups.

Abundance in Group 1: increased abundance in Children with diarrhea (CD)

NCBI Quality ControlLinks
Defluviitaleaceae
Eubacteriales
Gammaproteobacteria
Gemellaceae
Lachnospiraceae
Clostridiaceae_222000Clostridiaceae_222000
Enterobacterales_A_737866Enterobacterales_A_737866
StaphylococcalesStaphylococcales
[Clostridium] piliforme

Revision editor(s): Ameenatoloko

Signature 2

Reviewed Marked as Reviewed by KateRasheed on 2025-5-2

Curated date: 2025/04/30

Curator: Ameenatoloko

Revision editor(s): Ameenatoloko

Source: Fig. S4, Table S8

Description: A cladogram made by LEfSe demonstrates different bacterial taxa between the CD and HC groups.

Abundance in Group 1: decreased abundance in Children with diarrhea (CD)

NCBI Quality ControlLinks
Actinomycetales
Actinomycetes
Bacteroidia
Bifidobacteriaceae
Coriobacteriaceae
Coriobacteriales
Coriobacteriia
Eggerthellaceae
Bifidobacterium_388775Bifidobacterium_388775
Lachnospira
Blautia
Mediterraneibacter faecis
Bifidobacterium longum
Hungatella
Streptococcus vestibularis

Revision editor(s): Ameenatoloko