Faecal microbiota of schoolchildren is associated with nutritional status and markers of inflammation: a double-blinded cluster-randomized controlled trial using multi-micronutrient fortified rice/Experiment 5

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-2-7

Curated date: 2024/12/15

Curator: Joiejoie

Revision editor(s): Joiejoie, WikiWorks

Subjects

Location of subjects
Cambodia
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Nutritional deficiency disease malnourished,malnutrition,nutritional deficiency disease,Nutritional deficiency disease
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Placebo
Group 1 name Corresponds to the case (exposed) group for case-control studies
UR-Improved
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
This group includes children placed on the more diverse micronutrient-fortified rice (FORISCA) fortified with vitamins A, B3, and B12 as additional vitamins.
Group 0 sample size Number of subjects in the control (unexposed) group
114
Group 1 sample size Number of subjects in the case (exposed) group
122
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V5
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
arcsine square-root
Statistical test
MaAsLin2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2.0

Alpha Diversity

Pielou Quantifies how equal the community is numerically
unchanged
Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
unchanged

Signature 1

Needs review

Curated date: 2024/12/17

Curator: Joiejoie

Revision editor(s): Joiejoie, KateRasheed, WikiWorks

Source: Fig. 4

Description: Impact of the nutritional intervention on the taxonomic composition of the faecal microbiota of the 380 children

Abundance in Group 1: increased abundance in UR-Improved

NCBI Quality ControlLinks
Agathobacter
Blautia
Enterorhabdus
Muribaculaceae
Pediococcus
Prevotella_9Prevotella_9
Clostridium

Revision editor(s): Joiejoie, KateRasheed, WikiWorks

Signature 2

Needs review

Curated date: 2024/12/17

Curator: Joiejoie

Revision editor(s): Joiejoie, KateRasheed, WikiWorks

Source: Fig. 4

Description: Impact of the nutritional intervention on the taxonomic composition of the faecal microbiota of the 380 children

Abundance in Group 1: decreased abundance in UR-Improved

NCBI Quality ControlLinks
Alistipes
Allofournierella
Anaerobutyricum hallii
Anaerosalibacter
Anaerostipes
Bacteroides
Bilophila
Blautia
Butyricicoccus
Butyricimonas
Colidextribacter
Coprobacillaceae
Coprococcus
Desulfovibrio
Dorea
Faecalibacterium
Faecalibacterium sp. UBA1819
Firmicutes bacterium CAG:56
Fusicatenibacter
Intestinimonas
Lachnoclostridium
Lachnospira
Lachnospira eligens
Lachnospiraceae
Ligilactobacillus
Limosilactobacillus
Marvinbryantia
Odoribacter
Oscillospiraceae
Parabacteroides
Phascolarctobacterium
Ruminococcus gauvreauii
Rummeliibacillus
Sutterella
[Ruminococcus] torques
Erysipelotrichaceae.UCG.003Erysipelotrichaceae.UCG.003
Lachnospiraceae.ND3007.groupLachnospiraceae.ND3007.group
Lachnospiraceae.UCG.004Lachnospiraceae.UCG.004
UCG.005UCG.005
UCG.003UCG.003
Lachnospiraceae.FCS020.groupLachnospiraceae.FCS020.group
Incertae.SedisIncertae.Sedis
Holdemania

Revision editor(s): Joiejoie, KateRasheed, WikiWorks