Metformin modifies plasma microbial-derived extracellular vesicles in polycystic ovary syndrome with insulin resistance/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-6-11
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Blood plasma Blood plasm,Plasma,Portion of blood plasma,Portion of plasma,Blood plasma,blood plasma
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- EBF (EVs extracted from patients before treatment) group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- EAF (EVs extracted from patients after treatment) group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Polycystic ovary syndrome- Insulin-resistant patients of childbearing age, whose plasma EVs(extracellular vesicles) were extracted after 3 months of metformin treatment.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 5
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 6 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V9
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- PacBio RS
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-6-11
Source: Figure 6B
Description: Analysis of microbial species diversity. Microbial cladogram (LEfSe).
Abundance in Group 1: increased abundance in EAF (EVs extracted from patients after treatment) group
NCBI | Quality Control | Links |
---|---|---|
Mesorhizobium | ||
unclassified Mesorhizobium |
Revision editor(s): ShadeAkinremi, Victoria
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-6-11
Source: Figure 6B
Description: Analysis of microbial species diversity. Microbial cladogram (LEfSe).
Abundance in Group 1: decreased abundance in EAF (EVs extracted from patients after treatment) group
NCBI | Quality Control | Links |
---|---|---|
Sphingobacterium hotanense |
Revision editor(s): ShadeAkinremi, Victoria