Altitude-dependent agro-ecologies impact the microbiome diversity of scavenging indigenous chicken in Ethiopia/Experiment 3

From BugSigDB


Needs review

Curated date: 2025/02/22

Curator: KateRasheed

Revision editor(s): KateRasheed

Subjects

Location of subjects
Ethiopia
Host species Species from which microbiome was sampled. Contact us to have more species added.
Gallus gallus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Cecum mucosa Caecum mucosa,Caecum mucosa of organ,Caecum mucous membrane,Caecum organ mucosa,Cecal mucosa,Cecum mucosa of organ,Cecum mucous membrane,Cecum organ mucosa,Intestinum crassum caecum mucosa,Intestinum crassum caecum mucosa of organ,Intestinum crassum caecum mucous membrane,Intestinum crassum caecum organ mucosa,Mucosa of caecum,Mucosa of cecum,Mucosa of intestinum crassum caecum,Mucosa of organ of caecum,Mucosa of organ of cecum,Mucosa of organ of intestinum crassum caecum,Mucous membrane of caecum,Mucous membrane of cecum,Mucous membrane of intestinum crassum caecum,Organ mucosa of caecum,Organ mucosa of cecum,Organ mucosa of intestinum crassum caecum,Cecum mucosa,cecum mucosa
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Social environment measurement Social environment measurement,social environment measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Enterotype 1 + Enterotype 2
Group 1 name Corresponds to the case (exposed) group for case-control studies
Enterotype 3
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Enterotype 3 refers to the Ethiopian chicken caecal microbiota from the gene catalogue, that was classified under Enterotype 3. They were particularly distinct from enterotypes 1 and 2 and clearly dominated by Climate zone 1.
Group 1 sample size Number of subjects in the case (exposed) group
148

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
4


Signature 1

Needs review

Curated date: 2025/02/23

Curator: KateRasheed

Revision editor(s): KateRasheed

Source: Fig. 3C

Description: Differential enrichment of genera between enterotypes using LEfSe analysis

Abundance in Group 1: increased abundance in Enterotype 3

NCBI Quality ControlLinks
Prevotella
Megamonas
Faecalibacterium
Olsenella
Lactobacillus
Bifidobacterium
Mediterranea
Collinsella
Megasphaera
Dialister

Revision editor(s): KateRasheed