The impact of small intestinal bacterial overgrowth on the efficacy of fecal microbiota transplantation in patients with chronic constipation
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Wang L, Xu Y, Li L, Yang B, Zhao D, Ye C, Lin Z, Cui J, Liu Y, Zhu W, Li N, Tian H, Chen Q
Journal
mBio
Year
2024
Keywords:
chronic constipation, efficacy, fecal microbiota transplantation, small intestinal bacterial overgrowth
UNLABELLED: To investigate the impact of Small Intestinal Bacterial Overgrowth (SIBO) on the efficacy of Fecal Microbiota Transplantation (FMT) in patients with chronic constipation, our research team included 218 patients with chronic constipation treated with FMT. Based on the results of the SIBO breath test, the patients were divided into two groups: the constipation with SIBO group (SIBO) and the constipation without SIBO group (non-SIBO). The efficacy of the two groups was evaluated using constipation-related scoring scales. At the same time, feces and small intestinal fluid samples were collected from both groups before and after FMT to compare the changes in the intestinal microbiota through 16S rRNA sequencing. In this study, it was found that the clinical efficacy of FMT in the SIBO group was superior to that in the non-SIBO group. After FMT treatment, both groups showed a significant increase in bowel frequency and improvement in stool characteristics. Abdominal symptoms, rectal symptoms, and defecation symptoms were significantly alleviated (P < 0.05), and patients' quality of life was significantly enhanced (P < 0.05). After FMT, except for the Constipation Assessment Scale scores, other scale scores showed significant differences between the two groups, the SIBO group scoring significantly better than the non-SIBO group (P < 0.05). After FMT, there were minor changes in the colonic microbiota but more substantial changes in the small intestinal microbiota. At baseline, the SIBO group had a higher abundance of Veillonella, and lower abundances of Escherichia-Shigella and Acinetobacter compared to the non-SIBO group. Chronic constipation patients with SIBO have a better response to FMT than those without SIBO. IMPORTANCE: Existing studies have rarely considered the impact of the small intestine's microbial state on the efficacy of fecal microbiota transplantation (FMT), nor have they extensively explored the effect of the small intestine's microbial state on the recovery of colonic motility. Therefore, this study investigates the influence of small intestinal bacterial overgrowth (SIBO) on the efficacy of FMT in treating constipation, specifically the impact of the microbial state of the small intestine on the restoration of colonic homeostasis, and consequently on the recovery of colonic motility.
Experiment 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to transplant Response to transplant,response to transplant
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- PosF0 - Fecal sample in Small intestinal bacterial overgrowth group (SIBO group) at pre-FMT
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- PosF7 - Fecal sample in Small intestinal bacterial overgrowth group (SIBO group) seven days after FMT
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Small intestinal bacterial overgrowth (SIBO) group seven days after FMT
- Group 0 sample size Number of subjects in the control (unexposed) group
- 88
- Group 1 sample size Number of subjects in the case (exposed) group
- 88
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Extensive antibiotic use
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- -V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 3A
Description: Represents the LEfSe analysis of the colon microbiota and small intestinal microbiota for two groups of patients.
Abundance in Group 1: increased abundance in PosF7 - Fecal sample in Small intestinal bacterial overgrowth group (SIBO group) seven days after FMT
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidaceae | ||
| Bacteroidales | ||
| Bacteroidia | ||
| Bacteroidota | ||
| Bacteroides |
Revision editor(s): Miss Lulu
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 3A
Description: Represents the LEfSe analysis of the colon microbiota and small intestinal microbiota for two groups of patients.
Abundance in Group 1: decreased abundance in PosF7 - Fecal sample in Small intestinal bacterial overgrowth group (SIBO group) seven days after FMT
| NCBI | Quality Control | Links |
|---|---|---|
| Enterobacterales | ||
| Enterobacteriaceae | ||
| Gammaproteobacteria | ||
| Pseudomonadota | ||
| Escherichia/Shigella sp. |
Revision editor(s): Miss Lulu
Experiment 2
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Differences from previous experiment shown
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Small intestine Anterior intestine,Intestinum tenue,Mid intestine,Small bowel,Small intestine,small intestine
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- NegI7-small intestine sample in Small intestinal bacterial overgrowth group (Non-SIBO group) 7 days after FMT
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- POSI0-small intestine sample in Small intestinal bacterial overgrowth group (SIBO group) at pre-FMT
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Small intestinal bacterial overgrowth (SIBO) group before FMT
- Group 0 sample size Number of subjects in the control (unexposed) group
- 130
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 3D
Description: Represents the LEfSe analysis of the colon microbiota and small intestinal microbiota for two groups of patients.
Abundance in Group 1: decreased abundance in POSI0-small intestine sample in Small intestinal bacterial overgrowth group (SIBO group) at pre-FMT
| NCBI | Quality Control | Links |
|---|---|---|
| Enterobacterales | ||
| Enterobacteriaceae | ||
| Escherichia/Shigella sp. | ||
| Gammaproteobacteria | ||
| Pseudomonadota |
Revision editor(s): Miss Lulu
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 3D
Description: Changes in gut microbiota after FMT in two groups of patients: the SIBO group and the non-SIBO group
Abundance in Group 1: increased abundance in POSI0-small intestine sample in Small intestinal bacterial overgrowth group (SIBO group) at pre-FMT
| NCBI | Quality Control | Links |
|---|---|---|
| Bacilli | ||
| Bacillota | ||
| Lactobacillales | ||
| Streptococcaceae | ||
| Streptococcus |
Revision editor(s): Miss Lulu
Experiment 3
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- NegI0-small intestine sample in Small intestinal bacterial overgrowth group (Non-SIBO group) at pre-FMT
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 5A
Description: Gut microbiota differences between the small intestinal microbiota of two groups were identified with a LEfSe analysis with an LDA score threshold >2.0.
Abundance in Group 1: decreased abundance in POSI0-small intestine sample in Small intestinal bacterial overgrowth group (SIBO group) at pre-FMT
Revision editor(s): Miss Lulu
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 5A
Description: Gut microbiota differences between the small intestinal microbiota of two groups were identified with a LEfSe analysis with an LDA score threshold >2.0.
Abundance in Group 1: increased abundance in POSI0-small intestine sample in Small intestinal bacterial overgrowth group (SIBO group) at pre-FMT
| NCBI | Quality Control | Links |
|---|---|---|
| Deinococci | ||
| Deinococcota | ||
| Lachnospiraceae | ||
| Lachnospirales | ||
| Meiothermus | ||
| Sphingomonadaceae | ||
| Sphingomonadales | ||
| Sphingomonas | ||
| Thermaceae | ||
| Thermales |
Revision editor(s): Miss Lulu
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