Fecal microbiota transplantation from patients with polycystic ovary syndrome induces metabolic disorders and ovarian dysfunction in germ-free mice/Experiment 3
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-5-9
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Transplant outcome measurement Transplant outcome measurement,transplant outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Mice transplanted from the healthy controls on Day 28
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Mice transplanted from PCOS (Polycystic ovary syndrome) patients on Day 28
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The Mice transplanted from PCOS (Polycystic ovary syndrome) patients on Day 28 was given 100 µl of the fecal microbiota suspension from the PCOS patients.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3
Alpha Diversity
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-5-9
Source: Fig. 2D - 2E
Description: Results of LEfSe analysis and Cladogram showing bacterial taxa whose abundance in the gut significantly differed between D28-H and D28-P (lower and right).
Abundance in Group 1: increased abundance in Mice transplanted from PCOS (Polycystic ovary syndrome) patients on Day 28
| NCBI | Quality Control | Links |
|---|---|---|
| Lawsonibacter | ||
| Rikenellaceae | ||
| Phocaeicola_A_858004Phocaeicola_A_858004 | ||
| Mediterraneibacter_A_155507Mediterraneibacter_A_155507 | ||
| Oscillospiraceae_88309Oscillospiraceae_88309 |
Revision editor(s): PreciousChijioke
