Effects of healthy low-carbohydrate diet and time-restricted eating on weight and gut microbiome in adults with overweight or obesity: Feeding RCT/Experiment 6

From BugSigDB


Reviewed Marked as Reviewed by ChiomaBlessing on 2025-4-22

Curated date: 2025/04/14

Curator: Daberechi

Revision editor(s): Daberechi

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Fasting Fast,Fasting,fasting
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Time Restricted Eating at Baseline (TRE)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Time Restricted Eating at Endpoint (TRE)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
The participants in the Time Restricted Eating (TRE) alone group were provided with control diets and instructed to follow the 10-h TRE. The control diet was the traditional Chinese diet designed according to Dietary Guidelines for Chinese Resident characterized by approximately 50%, 34%, and 16% of energy from carbohydrates, fat, and protein. The 10-h TRE required participants to consume the provided meals within a 10-h window each day.
Group 0 sample size Number of subjects in the control (unexposed) group
48
Group 1 sample size Number of subjects in the case (exposed) group
43
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2025-4-22

Curated date: 2025/04/14

Curator: Daberechi

Revision editor(s): Daberechi

Source: Figure 2D and Supplementary Figure 2

Description: Significant taxonomic changes between Time Restricted Eating (TRE) at baseline and Endpoint as accessed using LefSe.

Abundance in Group 1: increased abundance in Time Restricted Eating at Endpoint (TRE)

NCBI Quality ControlLinks
Citrobacter
Enterobacter hormaechei
Enterobacterales
Enterobacteriaceae
Escherichia
Escherichia coli
Gammaproteobacteria
Klebsiella
Klebsiella pneumoniae
Parabacteroides
Parabacteroides distasonis
Tannerellaceae
Weissella confusa
Pseudomonadota

Revision editor(s): Daberechi

Signature 2

Reviewed Marked as Reviewed by ChiomaBlessing on 2025-4-22

Curated date: 2025/04/14

Curator: Daberechi

Revision editor(s): Daberechi

Source: Figure 2D and Supplementary Figure 2

Description: Significant taxonomic changes between Time Restricted Eating (TRE) at baseline and Endpoint as accessed using LefSe.

Abundance in Group 1: decreased abundance in Time Restricted Eating at Endpoint (TRE)

NCBI Quality ControlLinks
Clostridia
Coprococcus comes
Dorea
Dorea longicatena
Eubacterium ramulus
Faecalibacterium
Faecalibacterium prausnitzii
Mediterraneibacter
Peptostreptococcaceae
Romboutsia
Romboutsia timonensis
Streptococcus rubneri
Oscillospiraceae

Revision editor(s): Daberechi