Effects of healthy low-carbohydrate diet and time-restricted eating on weight and gut microbiome in adults with overweight or obesity: Feeding RCT/Experiment 8
From BugSigDB
Reviewed Marked as Reviewed by ChiomaBlessing on 2025-4-22
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to diet Response to diet,response to diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy Low Carbohydrate Diet at Baseline (HLCD)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Healthy Low Carbohydrate Diet at Endpoint (HLCD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The participants in the Healthy Low Carbohydrate Diet (HLCD) alone group were provided with HLCD and instructed to follow their usual eating regimens during the 12 week feeding trial. The HLCD was a relatively healthy type of LCD consisting of approximately 30%, 50%, and 20% of total energy from carbohydrates, fats, and proteins, emphasizing the consumption of unsaturated fatty acids, plant proteins, and high-quality carbohydrates including whole grains, fresh vegetables, and fruits.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 48
- Group 1 sample size Number of subjects in the case (exposed) group
- 43
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by ChiomaBlessing on 2025-4-22
Source: Supplementary Figure 3A
Description: Significant taxonomic changes between Healthy Low Carbohydrate Diet (HLCD) at baseline and Endpoint as accessed.
Abundance in Group 1: decreased abundance in Healthy Low Carbohydrate Diet at Endpoint (HLCD)
| NCBI | Quality Control | Links |
|---|---|---|
| Phocaeicola dorei | ||
| Ruminococcus callidus |
Revision editor(s): Daberechi
