Increase in body weight is lowered when mice received fecal microbiota transfer from donor mice treated with the AT1 receptor antagonist telmisartan/Experiment 1
Subjects
- Location of subjects
- Germany
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- d0 (day 0)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- d49 (day 49)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Stool samples collected at day 49 for microbiota analysis after the mice had already been fed High Fat Diet (HFD) for 7 weeks
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- log transformation
- Statistical test
- ANCOM-BC
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Source: Fig. S3B, S4B, S5B, Table S1
Description: Differential abundance analysis of phylum, family and genera between d0 and d49. At time point d0, the mice were still receiving chow diet, while d49 represents the time point immediately before FMT, when the two groups of mice had been fed HFD for 7 weeks but had not yet received microbiome transfer
Abundance in Group 1: increased abundance in d49 (day 49)
Revision editor(s): Anne-mariesharp
Signature 2
Source: Fig. S3B, S4B, S5B, Table S1
Description: Differential abundance analysis of phylum, family and genera between d0 and d49. At time point d0, the mice were still receiving chow diet, while d49 represents the time point immediately before FMT, when the two groups of mice had been fed HFD for 7 weeks but had not yet received microbiome transfer
Abundance in Group 1: decreased abundance in d49 (day 49)
Revision editor(s): Anne-mariesharp, KateRasheed