The regulatory effect of chitooligosaccharides on islet inflammation in T2D individuals after islet cell transplantation: the mechanism behind Candida albicans abundance and macrophage polarization/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-4-3

Curated date: 2025/03/26

Curator: Tosin

Revision editor(s): Tosin

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to transplant Response to transplant,response to transplant
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Con (Control) group
Group 1 name Corresponds to the case (exposed) group for case-control studies
ICT (islet cell transplantation) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients with T2D (type 2 diabetes) who underwent ICT (islet cell transplantation)
Group 0 sample size Number of subjects in the control (unexposed) group
16
Group 1 sample size Number of subjects in the case (exposed) group
33
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
Current use of antibiotics

Lab analysis

Sequencing type
18S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
decreased
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Richness Number of species
decreased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-3

Curated date: 2025/03/26

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 2I

Description: Differential abundant taxa between CON (control) and ICT (islet cell transplantation) groups by LeFse.

Abundance in Group 1: increased abundance in ICT (islet cell transplantation) group

NCBI Quality ControlLinks
Amphisiella
Amphisiella magnigranulosa
Candida
Candida albicans
Candida dubliniensis
Cystofilobasidiaceae
Cystofilobasidium
Debaryomycetaceae
Malassezia globosa
Nakaseomyces glabratus

Revision editor(s): Tosin

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-4-3

Curated date: 2025/03/26

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 2I

Description: Differential abundant taxa between CON (control) and ICT (islet cell transplantation) groups by LeFse.

Abundance in Group 1: decreased abundance in ICT (islet cell transplantation) group

NCBI Quality ControlLinks
Hannaella luteola
Imbricatea
Leucosporidiaceae
Leucosporidiales
Leucosporidium
Leucosporidium yakuticum
Polygonaceae
Rheum
Rheum spiciforme

Revision editor(s): Tosin