The regulatory effect of chitooligosaccharides on islet inflammation in T2D individuals after islet cell transplantation: the mechanism behind Candida albicans abundance and macrophage polarization/Experiment 3
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- RA (Rapamycin alone) treatment group of HMA-T2D (Human Microbiota associated type 2 diabetes) mice
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- RC (Rapamycin + Chitooligosaccharides ) treatment group of HMA-T2D (Human Microbiota associated type 2 diabetes) mice
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The HMA-T2D (Human Microbiota associated type 2 diabetes) mice gavaged with Rapamycin (RAPA) 5 mg/kg every other day and Chitooligosaccharides (COS) 350 mg/kg/day.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
Lab analysis
- Sequencing type
- 18S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Richness Number of species
- unchanged
Signature 1
Source: Figure 8B and 8C
Description: Analysis of the gut fungal community abundance differences between the RA (Rapamycin alone) and RC (Rapamycin + Chitooligosaccharides) groups of HMA-T2D (Human Microbiota associated type 2 diabetes) mice by LEfSe analysis
Abundance in Group 1: increased abundance in RC (Rapamycin + Chitooligosaccharides ) treatment group of HMA-T2D (Human Microbiota associated type 2 diabetes) mice
Revision editor(s): Tosin
Signature 2
Source: Figure 8B and 8C
Description: Analysis of the gut fungal community abundance differences between the RA (Rapamycin alone) and RC (Rapamycin + Chitooligosaccharides) groups of HMA-T2D (Human Microbiota associated type 2 diabetes) mice by LEfSe analysis
Abundance in Group 1: decreased abundance in RC (Rapamycin + Chitooligosaccharides ) treatment group of HMA-T2D (Human Microbiota associated type 2 diabetes) mice
| NCBI | Quality Control | Links |
|---|---|---|
| Arxiozyma telluris | ||
| Candida albicans | ||
| Hanseniaspora | ||
| Hanseniaspora uvarum | ||
| Kazachstania | ||
| Mattesia | ||
| unclassified Mattesia |
Revision editor(s): Tosin
