New insights into the combined effects of aflatoxin B1 and Eimeria ovinoidalis on uterine function by disrupting the gut-blood-reproductive axis in sheep/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-3-28

Curated date: 2025/03/24

Curator: Tosin

Revision editor(s): Tosin

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Ovis aries
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Combination of Con(control), AFB1 (Aflatoxin B1), AFB1 + E0 (Aflatoxin B1 + Eimeria ovinoidalis) treatment groups
Group 1 name Corresponds to the case (exposed) group for case-control studies
E.0 (Eimeria ovinoidalis) treatment group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
A treatment group with E.0 (Eimeria ovinoidalis) which is a coccidian parasite that has been identified as the most pathogenic species among sheep worldwide and received a single oral administration of 2 × 104 sporulated oocysts on day 0 only.
Group 0 sample size Number of subjects in the control (unexposed) group
12
Group 1 sample size Number of subjects in the case (exposed) group
4

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2


Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-3-28

Curated date: 2025/03/24

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 3f

Description: Linear discriminant analysis effect size (LEfSe) comparison analysis between the groups.

Abundance in Group 1: increased abundance in E.0 (Eimeria ovinoidalis) treatment group

NCBI Quality ControlLinks
Bacteroidales
Bacteroidia
Bacteroidota
Pseudomonadota
Gammaproteobacteria

Revision editor(s): Tosin