New insights into the combined effects of aflatoxin B1 and Eimeria ovinoidalis on uterine function by disrupting the gut-blood-reproductive axis in sheep/Experiment 2

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-3-28

Curated date: 2025/03/24

Curator: Tosin

Revision editor(s): Tosin

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Ovis aries
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Combination of Con(control), E.0 (Eimeria ovinoidalis), AFB1 + E0 (Aflatoxin B1 + Eimeria ovinoidalis) treatment groups
Group 1 name Corresponds to the case (exposed) group for case-control studies
AFB1 (Aflatoxin B1) treatment group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Treatment group with Aflatoxin B1 which is a mycotoxin produced by Aspergillus flavus and Aspergillus parasiticus, is a member of the aflatoxin family and is commonly found in cereals and animal feed all over the world, together with other fungal toxins and received daily oral administration of AFB1 at a dosage of 60 μg/kg
Group 0 sample size Number of subjects in the control (unexposed) group
12
Group 1 sample size Number of subjects in the case (exposed) group
4

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2


Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-3-28

Curated date: 2025/03/24

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 3f

Description: Linear discriminant analysis effect size (LEfSe) comparison analysis between the groups.

Abundance in Group 1: increased abundance in AFB1 (Aflatoxin B1) treatment group

NCBI Quality ControlLinks
Clostridia
Eubacteriales
Oscillospiraceae
Verrucomicrobiales
Verrucomicrobiia
Verrucomicrobiota
Akkermansia
Verrucomicrobiaceae

Revision editor(s): Tosin