Altered microbiome and metabolome profiling in fearful companion dogs: An exploratory study/Experiment 1
From BugSigDB
Needs review
Curated date: 2025/02/15
Curator: Akritishakya
Revision editor(s): Akritishakya, WikiWorks, Fiddyhamma
Subjects
- Location of subjects
- Italy
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Canis lupus familiaris
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Mental or behavioural disorder Behavior Disorder,Brief Reactive Psychoses,Brief Reactive Psychosis,disease of mental health,disorder of mental process,Disorder, Psychotic,Disorders, Psychotic,Mental Disorder,mental disorder,mental disorders,mental or behavioral disorder,mental or behavioural disorder,mental process disease,Psychiatric Disorder,Psychoses,Psychoses, Brief Reactive,Psychosis, Brief Reactive,PSYCHOTIC DIS,Psychotic Disorder,Psychotic Disorders,Reactive Psychoses, Brief,Reactive Psychosis, Brief,Mental or behavioural disorder
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy Control
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Fearful Dogs
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Dogs exhibiting generalized fear, assessed through behavioral tests and owner-reported questionnaries.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4-V6
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- edgeR
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Needs review
Source: Figure 3
Description: Significantly different taxa identified between case and control groups by EdgeR analysis
Abundance in Group 1: increased abundance in Fearful Dogs
| NCBI | Quality Control | Links |
|---|---|---|
| Coprobacillaceae | ||
| Peptostreptococcales TissierellalesPeptostreptococcales Tissierellales |
Revision editor(s): Akritishakya, Fiddyhamma
Signature 2
Needs review
Source: Figure 3
Description: Significantly different taxa identified between case and control groups by EdgeR analysis
Abundance in Group 1: decreased abundance in Fearful Dogs
| NCBI | Quality Control | Links |
|---|---|---|
| Gammaproteobacteria | ||
| Dorea |
Revision editor(s): Fiddyhamma
