Characteristics of functional constipation and analysis of intestinal microbiota in children aged 0-4 in Zunyi region
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Gong S, Yu Z, Ding Y, Wang Y, Li X, Gu S
Journal
BMC pediatrics
Year
2025
Keywords:
Children, Functional constipation, Gut microbiota, Rome IV criteria, Zunyi
BACKGROUND: Functional constipation (FC) significantly impacts children's health. This study investigates the prevalence and microbiota characteristics of FC in children aged 0-4 years in Zunyi area. METHODS: From October to December 2023, 2039 children aged 0-4 years in Zunyi were selected using stratified sampling and cross-sectional survey methods. A questionnaire based on Rome IV diagnostic criteria was used. Twenty-nine children with FC were randomly selected as the functional constipation group (FCG), and 26 healthy children, matched for age, sex, and area, were selected as the control group (CG). RESULTS: A total of 2051 questionnaires were collected, with 2039 valid responses. Among them, 151 children had FC, resulting in a prevalence rate of 7.4%. The prevalence rates in boys and girls were 6.6% and 8.5%, respectively, with no significant gender difference (P > 0.05). Alpha diversity analysis revealed higher richness and diversity of intestinal flora in the FCG compared to the CG. At the phylum level, Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes were dominant in both groups. The FCG showed a higher relative abundance of Firmicutes, Actinobacteria, and Proteobacteria compared to the CG (P < 0.05). CONCLUSIONS: The prevalence of FC in children aged 0-4 years in Zunyi is 7.4%. Disease characteristics vary with age and living environment but are unrelated to gender. The gut microbiota of children with FC shows significant alterations, with higher diversity and specific phyla abundance.
Experiment 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-21
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Chronic constipation Chronic constipation,Infrequent bowel movements,chronic constipation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control group (CG)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Functional constipation group (FCG)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Individuals who have been diagnosed with the functional constipation(FCG). Functional constipation (FC) is a common pediatric gastrointestinal disorder causing infrequent and dry stools.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 26
- Group 1 sample size Number of subjects in the case (exposed) group
- 29
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- Matched on Factors on which subjects have been matched on in a case-control study
- age, region, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-21
Source: Fig 7&8
Description: Differences in relative abundance of species at the phylum and genus level between two groups
Abundance in Group 1: increased abundance in Functional constipation group (FCG)
Revision editor(s): Aiyshaaaa
Signature 3
Reviewed Marked as Reviewed by Svetlana up on 2025-3-21
Source: Fig 7&8
Description: Differences in relative abundance of species at the phylum and genus level between two groups
Abundance in Group 1: decreased abundance in Functional constipation group (FCG)
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium | ||
| Lactobacillus | ||
| Streptococcus | ||
| Anaerobutyricum hallii | ||
| Actinomycetota |
Revision editor(s): Aiyshaaaa
Experiment 2
Reviewed Marked as Reviewed by Svetlana up on 2025-3-21
Differences from previous experiment shown
Subjects
Lab analysis
Statistical Analysis
- Statistical test
- LEfSe
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 4
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-21
Source: Fig 9
Description: LEfSe multi-level species difference discriminant analysis between two groups in Phylum,Class,Order,Family and Genus level
Abundance in Group 1: increased abundance in Functional constipation group (FCG)
Revision editor(s): Aiyshaaaa
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-3-21
Source: Fig 9
Description: LEfSe multi-level species difference discriminant analysis between two groups in Phylum,Class,Order,Family and Genus level
Abundance in Group 1: decreased abundance in Functional constipation group (FCG)
Revision editor(s): Aiyshaaaa
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