Dysbiosis of infant gut microbiota is related to the altered fatty acid composition of human milk from mothers with gestational diabetes mellitus: a prospective cohort study

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Reviewed Marked as Reviewed by Svetlana up on 2025-3-26
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Li K, Jin J, Liu Z, Chen C, Huang L, Sun Y
Journal
Gut microbes
Year
2025
Keywords:
Gut microbiota, gestational diabetes mellitus, human milk, infant, lactation
Gestational diabetes mellitus (GDM) is known to be associated with dysbiosis of offspring gut microbiota, but the mechanism remains unclear. The present prospective study explored the role of human milk fatty acid composition in this association. Mothers with GDM and normal controls were recruited at 24-28 gestational weeks. Follow-up was conducted at 1-3 days postpartum and 1 month postpartum to collect human milk and infant feces. A total of 80 mother-infant pairs (40 in the GDM group and 40 in the normal group) were included in the study. The mothers received guidance on diet and exercise but not drug therapy. All infants were exclusively breastfed. We observed significant differences in 8 phyla and 13 genera in the infant between GDM and normal groups at 1-3 days postpartum or 1 month postpartum. Among these bacteria, significant time × group interaction was observed for 7 phyla (such as Acidobacteriota, Gemmatimonadota, and Myxococcota) and 9 genera (such as Sphingomonas, Allorhizobium Neorhizobium Pararhizobium Rhizobium, and TM7a), after adjusting for confounding factors. Changes in these differential infant bacteria were negatively correlated with changes in C18:3n-3 and total n-3 PUFA levels of breast milk. The increases in C18:3n-3 and total n-3 PUFA levels in human milk over time were much greater in the normal group compared to the GDM group. Our findings indicate that altered human milk fatty acid composition is one important reason for GDM-related dysbiosis of offspring gut microbiota.

Experiment 1


Reviewed Marked as Reviewed by Svetlana up on 2025-3-26

Curated date: 2025/03/19

Curator: Tosin

Revision editor(s): Tosin

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colostrum Colostrum,colostrum
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Gestational diabetes diabetes in pregnancy,GDM,gestational diabetes,gestational diabetes mellitus,maternal gestational diabetes mellitus,Gestational diabetes
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Normal group 0 (1-3 days after delivery)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Gestational diabetes mellitus group 0 (GDM0) (1-3 days after delivery)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Pregnant women with GDM (gestational diabetes mellitus) 1-3 days postpartum
Group 0 sample size Number of subjects in the control (unexposed) group
48
Group 1 sample size Number of subjects in the case (exposed) group
45
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Welch's T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-3-26

Curated date: 2025/03/19

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 5A and 5B

Description: Differential bacteria between Gestational diabetes mellitus (GDM) and normal groups at levels of phylum (A) and genus (B) at 1–3 days postpartum [Gestational diabetes mellitus0 (GDM0) and Normal0]

Abundance in Group 1: decreased abundance in Gestational diabetes mellitus group 0 (GDM0) (1-3 days after delivery)

NCBI Quality ControlLinks
Acidobacteriota
Candidatus Methylomirabilota
Candidatus Sumerlaeota
Dongia
Gemmatimonadota
Myxococcota
Nitrospirota
Sphingobacterium
unclassified Sphingobacteriaceae
Unclassified_Subgroup_7Unclassified_Subgroup_7
Unclassified_LWQ8Unclassified_LWQ8
Candidatus Altimarinota

Revision editor(s): Tosin

Experiment 2


Reviewed Marked as Reviewed by Svetlana up on 2025-3-26

Curated date: 2025/03/19

Curator: Tosin

Revision editor(s): Tosin

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Milk Mammary gland milk,Milk,milk
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Normal group 1 (1 month postpartum)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Gestational diabetes mellitus group 1 (GDM1) (1 month postpartum)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Pregnant women with GDM (gestational diabetes mellitus) 1 month postpartum
Group 0 sample size Number of subjects in the control (unexposed) group
40
Group 1 sample size Number of subjects in the case (exposed) group
40

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-3-26

Curated date: 2025/03/19

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 5A and 5B

Description: Differential bacteria between Gestational diabetes mellitus (GDM) and normal groups at levels of phylum (A) and genus (B) at 1 month postpartum [Gestational diabetes mellitus1 (GDM1) and Normal1]

Abundance in Group 1: increased abundance in Gestational diabetes mellitus group 1 (GDM1) (1 month postpartum)

NCBI Quality ControlLinks
Brevundimonas
Candidatus Altimarinota
Cyanobacteriota
Sphingomonas
unclassified Burkholderiales
unclassified Candidatus Saccharibacteria
unclassified Paracoccaceae
unclassified Sphingobacteriaceae
Unclassified_CyanobaterialesUnclassified_Cyanobateriales
Unclassified_LWQ8Unclassified_LWQ8
Allorhizobium_Neorhizobium_Pararhizobium_RhizobiumAllorhizobium_Neorhizobium_Pararhizobium_Rhizobium
Burkholderia_Caballeronia_ParaburkholderiaBurkholderia_Caballeronia_Paraburkholderia

Revision editor(s): Tosin

Experiment 3


Reviewed Marked as Reviewed by Svetlana up on 2025-3-26

Curated date: 2025/03/19

Curator: Tosin

Revision editor(s): Tosin

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colostrum Colostrum,colostrum
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Normal group 0 (1-3 days after delivery)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Gestational diabetes mellitus group 0 (GDM) (1-3 days after delivery)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Pregnant women with GDM (gestational diabetes mellitus) 1-3 days postpartum
Group 0 sample size Number of subjects in the control (unexposed) group
48
Group 1 sample size Number of subjects in the case (exposed) group
45

Lab analysis

Statistical Analysis

Statistical test
Linear Regression
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
birth weight, body mass index, Confounders controlled for: "birth length" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.birth length, Confounders controlled for: "maternal dietary carbohydrate" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.maternal dietary carbohydrate, Confounders controlled for: "dietary fibre intake" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.dietary fibre intake

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-3-26

Curated date: 2025/03/19

Curator: Tosin

Revision editor(s): Tosin

Source: Table 3

Description: Differential bacteria between GDM and normal groups with a p-time×group interaction < 0.05.

Abundance in Group 1: increased abundance in Gestational diabetes mellitus group 0 (GDM) (1-3 days after delivery)

NCBI Quality ControlLinks
unclassified Paracoccaceae

Revision editor(s): Tosin

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-3-26

Curated date: 2025/03/26

Curator: Tosin

Revision editor(s): Tosin

Source: Table 3

Description: Differential bacteria between GDM and normal groups with a p-time×group interaction < 0.05.

Abundance in Group 1: decreased abundance in Gestational diabetes mellitus group 0 (GDM) (1-3 days after delivery)

NCBI Quality ControlLinks
Acidobacteriota
Candidatus Altimarinota
Candidatus Methylomirabilota
Candidatus Sumerlaeota
Dongia
Gemmatimonadota
Myxococcota
Nitrospirota
Sphingomonas
Allorhizobium_Neorhizobium_Pararhizobium_RhizobiumAllorhizobium_Neorhizobium_Pararhizobium_Rhizobium
unclassified Candidatus Saccharibacteria
Unclassified Subgroup 7Unclassified Subgroup 7
unclassified Sphingobacteriaceae
Sphingobacterium
Unclassified LWQ8Unclassified LWQ8

Revision editor(s): Tosin

Experiment 4


Reviewed Marked as Reviewed by Svetlana up on 2025-3-26

Curated date: 2025/03/26

Curator: Tosin

Revision editor(s): Tosin

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Milk Mammary gland milk,Milk,milk
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Normal group 1 (1 month postpartum)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Gestational diabetes mellitus group 1 (GDM) (1 month postpartum)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Pregnant women with GDM (gestational diabetes mellitus) 1 month postpartum
Group 0 sample size Number of subjects in the control (unexposed) group
40
Group 1 sample size Number of subjects in the case (exposed) group
40

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-3-26

Curated date: 2025/03/26

Curator: Tosin

Revision editor(s): Tosin

Source: Table 3

Description: Differential bacteria between GDM and normal groups with a p-time×group interaction < 0.05.

Abundance in Group 1: increased abundance in Gestational diabetes mellitus group 1 (GDM) (1 month postpartum)

NCBI Quality ControlLinks
Candidatus Altimarinota
Sphingomonas
unclassified Sphingobacteriaceae
Allorhizobium_Neorhizobium_Pararhizobium_RhizobiumAllorhizobium_Neorhizobium_Pararhizobium_Rhizobium
unclassified Candidatus Saccharibacteria
Unclassified LWQ8Unclassified LWQ8
unclassified Paracoccaceae

Revision editor(s): Tosin