A legume-enriched diet improves metabolic health in prediabetes mediated through gut microbiome: a randomized controlled trial
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- Singapore
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Diet measurement Diet measurement,diet measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control Group (CG)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Intervention Group (IG)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group consists of participants who received the legume-enriched, calorie-restricted intervention diet, high in fiber and low-GI carbs, with legumes as the main protein source.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 57
- Group 1 sample size Number of subjects in the case (exposed) group
- 54
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- centered log-ratio
- Statistical test
- Linear Regression
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Signature 1
Source: Fig. 3F & S4a
Description: Taxa that are significantly changed over time in intervention.
Abundance in Group 1: increased abundance in Intervention Group (IG)
Revision editor(s): Joiejoie
Signature 2
Source: Fig. 3F & S4b
Description: Taxa that are significantly changed over time in intervention group.
Abundance in Group 1: decreased abundance in Intervention Group (IG)
Revision editor(s): Joiejoie
Experiment 2
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Day 0 - Control (ctr)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 1 - Control (ctr)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This is the Day 1 timepoint within the control (ctr) group
- Group 0 sample size Number of subjects in the control (unexposed) group
- Not specified
- Group 1 sample size Number of subjects in the case (exposed) group
- Not specified
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Source: Fig. S6
Description: Species that changed significantly in in vitro fermentation experiments
Abundance in Group 1: decreased abundance in Day 1 - Control (ctr)
NCBI | Quality Control | Links |
---|---|---|
Agathobacter rectalis | ||
Ruminococcus bromii |
Revision editor(s): Joiejoie
Experiment 3
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 2 - Control (ctr)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This is the Day 2 timepoint within the control (ctr) group
Lab analysis
Statistical Analysis
Signature 1
Source: Fig. S6
Description: Species that changed significantly in in vitro fermentation experiments
Abundance in Group 1: decreased abundance in Day 2 - Control (ctr)
NCBI | Quality Control | Links |
---|---|---|
Agathobacter rectalis | ||
Ruminococcus bromii |
Revision editor(s): Joiejoie
Experiment 4
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Day 0 - Red bean fibre digested (RBD)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 1 -Red bean fibre digested (RBD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This is the Day 1 time point within the Red bean fibre digested (RBD) group.
Lab analysis
Statistical Analysis
Signature 1
Source: Fig. S6
Description: Species that changed significantly in in vitro fermentation experiments
Abundance in Group 1: decreased abundance in Day 1 -Red bean fibre digested (RBD)
NCBI | Quality Control | Links |
---|---|---|
Agathobacter rectalis | ||
Ruminococcus bromii |
Revision editor(s): Joiejoie
Experiment 5
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 2 - Red bean fibre digested (RBD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This is the Day 2 time point within the Red bean fibre digested (RBD) group.
Lab analysis
Statistical Analysis
Signature 1
Source: Fig. S6
Description: Species that changed significantly in in vitro fermentation experiments
Abundance in Group 1: decreased abundance in Day 2 - Red bean fibre digested (RBD)
NCBI | Quality Control | Links |
---|---|---|
Agathobacter rectalis |
Revision editor(s): Joiejoie
Experiment 6
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Day 1 - Red bean fibre digested (RBD)
Lab analysis
Statistical Analysis
Signature 1
Source: Fig. S6
Description: Species that changed significantly in in vitro fermentation experiments
Abundance in Group 1: increased abundance in Day 2 - Red bean fibre digested (RBD)
NCBI | Quality Control | Links |
---|---|---|
Ruminococcus bromii |
Revision editor(s): Joiejoie
Experiment 7
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Day 0 - Red bean fibre undigested (RBU)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 1 - Red bean fibre undigested (RBU)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This is the Day 1 time point within the Red bean fibre undigested (RBU) group.
Lab analysis
Statistical Analysis
Signature 1
Source: Fig. S6
Description: Species that changed significantly in in vitro fermentation experiments
Abundance in Group 1: decreased abundance in Day 1 - Red bean fibre undigested (RBU)
NCBI | Quality Control | Links |
---|---|---|
Agathobacter rectalis | ||
Ruminococcus bromii |
Revision editor(s): Joiejoie
Experiment 8
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 2 - Red bean fibre undigested (RBU)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This is the Day 2 time point within the Red bean fibre undigested (RBU) group.
Lab analysis
Statistical Analysis
Signature 1
Source: Fig. S6
Description: Species that changed significantly in in vitro fermentation experiments
Abundance in Group 1: decreased abundance in Day 2 - Red bean fibre undigested (RBU)
NCBI | Quality Control | Links |
---|---|---|
Agathobacter rectalis |
Revision editor(s): Joiejoie
Experiment 9
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Day 1 - Red bean fibre undigested (RBU)
Lab analysis
Statistical Analysis
Signature 1
Source: Fig. S6
Description: Species that changed significantly in in vitro fermentation experiments
Abundance in Group 1: increased abundance in Day 2 - Red bean fibre undigested (RBU)
NCBI | Quality Control | Links |
---|---|---|
Ruminococcus bromii |
Revision editor(s): Joiejoie
Experiment 10
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Day 0 - Brown rice fibre digested (BRD)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 1 - Brown rice fibre digested (BRD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This is the Day 1 time point within the Brown rice fibre digested (BRD) group.
Lab analysis
Statistical Analysis
Signature 1
Source: Fig. S6
Description: Species that changed significantly in in vitro fermentation experiments
Abundance in Group 1: decreased abundance in Day 1 - Brown rice fibre digested (BRD)
NCBI | Quality Control | Links |
---|---|---|
Agathobacter rectalis | ||
Ruminococcus bromii |
Revision editor(s): Joiejoie
Experiment 11
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 2 - Brown rice fibre digested (BRD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This is the Day 2 time point within the Brown rice fibre digested (BRD) group.
Lab analysis
Statistical Analysis
Signature 1
Source: Fig. S6
Description: Species that changed significantly in in vitro fermentation experiments
Abundance in Group 1: decreased abundance in Day 2 - Brown rice fibre digested (BRD)
NCBI | Quality Control | Links |
---|---|---|
Agathobacter rectalis | ||
Ruminococcus bromii |
Revision editor(s): Joiejoie
Experiment 12
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Day 0 - Brown rice fibre undigested (BRU)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 1 - Brown rice fibre undigested (BRU)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This is the Day 1 time point within the Brown rice fibre undigested (BRU) group.
Lab analysis
Statistical Analysis
Signature 1
Source: Fig. S6
Description: Species that changed significantly in in vitro fermentation experiments
Abundance in Group 1: decreased abundance in Day 1 - Brown rice fibre undigested (BRU)
NCBI | Quality Control | Links |
---|---|---|
Agathobacter rectalis | ||
Ruminococcus bromii |
Revision editor(s): Joiejoie
Experiment 13
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 2 - Brown rice fibre undigested (BRU)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This is the Day 2 time point within the Brown rice fibre undigested (BRU) group.
Lab analysis
Statistical Analysis
Signature 1
Source: Fig. S6
Description: Species that changed significantly in in vitro fermentation experiments
Abundance in Group 1: decreased abundance in Day 2 - Brown rice fibre undigested (BRU)
NCBI | Quality Control | Links |
---|---|---|
Agathobacter rectalis | ||
Ruminococcus bromii |
Revision editor(s): Joiejoie