Gut microbiota modulation via fecal microbiota transplantation mitigates hyperoxaluria and calcium oxalate crystal depositions induced by high oxalate diet/Experiment 6

From BugSigDB


Needs review

Curated date: 2025/04/05

Curator: Dorcas-od

Revision editor(s): Dorcas-od, Anne-mariesharp

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Rattus norvegicus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to transplant Response to transplant,response to transplant
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Normal Group (45 days)
Group 1 name Corresponds to the case (exposed) group for case-control studies
5%K₂OxFMT (45 days)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Rats exposed to a 5%K₂Ox diet and treated with fecal microbiota transplantation (FMT) from healthy rat donors every two days for 45 days
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
ANOVA
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
decreased

Signature 1

Needs review

Curated date: 2025/04/05

Curator: Dorcas-od

Revision editor(s): Dorcas-od

Source: Figure 4a

Description: The effects of FMT on gut microbiota and metabolism.

Abundance in Group 1: increased abundance in 5%K₂OxFMT (45 days)

NCBI Quality ControlLinks
Actinomycetota
Bacteroidota
Pseudomonadota

Revision editor(s): Dorcas-od

Signature 2

Needs review

Curated date: 2025/04/05

Curator: Dorcas-od

Revision editor(s): Dorcas-od

Source: Figure 4a

Description: The effects of FMT on gut microbiota and metabolism.

Abundance in Group 1: decreased abundance in 5%K₂OxFMT (45 days)

NCBI Quality ControlLinks
Bacillota

Revision editor(s): Dorcas-od