Fecal microbiota transplantation modulates jejunal host-microbiota interface in weanling piglets/Experiment 2
From BugSigDB
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Sus scrofa domesticus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Jejunum Intestinum jejunum,Mid-intestine,Middle intestine,Jejunum,jejunum
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Clinical treatment Clinical treatment,clinical treatment
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Enterotoxigenic Escherichia coli (ETEC) challenge control group (EC)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- FMT-EC challenge group (FMT + EC)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Enterotoxigenic Escherichia coli challenge (ETEC) group that received fecal microbiota preparation from 3-month-old healthy pigs on the 1st and 3rd day after weaning.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.1
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Needs review
Source: Figure 9F, Supplementary Material 3
Description: Bacterial genera differentially abundant in ETEC-infected control piglets (EC) and ETEC-infected piglets that received prior FMT treatment (FMT-EC).
Abundance in Group 1: increased abundance in FMT-EC challenge group (FMT + EC)
Revision editor(s): Taofeecoh, Aleru Divine
Signature 2
Source: Figure 9F, Supplementary Material 3
Description: Bacterial genera differentially abundant in ETEC-infected control piglets (EC) and ETEC-infected piglets that received prior FMT treatment (FMT-EC).
Abundance in Group 1: decreased abundance in FMT-EC challenge group (FMT + EC)
Revision editor(s): Taofeecoh
