Fecal microbiota transplantation modulates jejunal host-microbiota interface in weanling piglets/Experiment 3
From BugSigDB
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Sus scrofa domesticus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Jejunum Intestinum jejunum,Mid-intestine,Middle intestine,Jejunum,jejunum
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Clinical treatment Clinical treatment,clinical treatment
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Fecal microbiota transplantation (FMT) group.
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- 3-week-old piglets that received fecal microbiota preparation from 3-month-old healthy pigs on the 1st and 3rd day after weaning.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.1
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
Signature 1
Source: Figures 1B, 2C, Supplementary Material 3
Description: Differentially abundant taxa between control and Fecal microbiota transplantation (FMT) groups.
Abundance in Group 1: increased abundance in Fecal microbiota transplantation (FMT) group.
Revision editor(s): Taofeecoh
Signature 2
Source: Figures 1B, 2C, Supplementary Material 3
Description: Differentially abundant taxa between control and Fecal microbiota transplantation (FMT) groups.
Abundance in Group 1: decreased abundance in Fecal microbiota transplantation (FMT) group.
Revision editor(s): Taofeecoh
