Different Efficacy of Five Soluble Dietary Fibers on Alleviating Loperamide-Induced Constipation in Mice: Influences of Different Structural Features
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Zhang Z, Liu B, Liu W, Liu X, Zhang C, Hu W, Wu W
Journal
International journal of molecular sciences
Year
2025
Keywords:
constipation, defecation function, gut microbiota, short-chain fatty acid, soluble dietary fiber, structural difference
Different dietary fibers have distinct structures, leading to significant variations in their laxative effects. To explore how these structural differences impact constipation intervention, a 14-day study was conducted on loperamide-induced constipated mice using five dietary fibers: soluble dietary fiber from steamed sweet potato (SDF-S), oat β-glucan (OB), polydextrose (PD), arabinogalactan (AG), and inulin (IN). The results showed that four fibers, excluding PD, significantly improved gastrointestinal (GI) transit rate (p < 0.05), although PD had the highest fecal moisture, it was significantly different from the lowest IN (p < 0.05). AG and IN resulted in higher 6 h fecal weights compared to other fibers. SDF-S and OB were more effective in modulating serum levels of gastrointestinal hormones. The different monosaccharide compositions and glycosidic bonds of these fibers led to distinct changes in gut microbiota composition and SCFA profiles. Galactose and arabinose in AG were linked to increased abundance of Lachnospiraceae_UCG-006, Bacteroides, and Odoribacter, promoting butyrate fermentation, which is positively correlated with GI transit rate. Glucose in SDF-S, OB, and PD favored acetate fermentation positively correlated with fecal moisture. Fructose in IN encouraged the proliferation of Muribaculaceae_unclassified and Ruminococcus, associated with butyrate fermentation and increased 6 h stool weight, respectively. The β-glycosidic bonds in OB may lead to high butyrate production through the selective proliferation of Lachnospiraceae_unclassified. Minor components like fucose, rhamnose, and ribose were positively correlated with the abundance of Oscillospiraceae_unclassified, Anaerotignum, and Lachnospiraceae_unclassified. In conclusion, the unique monosaccharide compositions and glycosidic bond differences in dietary fibers selectively promote the proliferation of fiber-degrading and butyrate-producing bacteria, resulting in varied effects on constipation relief.
Experiment 1
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Caecum Blind intestine,Blindgut,Caeca,Caecum,Ceca,Cecum,Intestinum caecum,Intestinum crassum caecum,Intestinum crassum cecum,caecum
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to diet Response to diet,response to diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Model control group (MC)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Steamed sweet potato group (SDF-S)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Male ICR mice administered Steamed sweet potato (SDF-S) the corresponding soluble fibers at doses of 400 mg/kg bw/d
- Group 0 sample size Number of subjects in the control (unexposed) group
- 12
- Group 1 sample size Number of subjects in the case (exposed) group
- 12
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Kruskall-Wallis
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Source: Figures 4A,D,E,G,H,I,J-L
Description: Relative Abundance of genera in the gut microbiota of mice
Abundance in Group 1: increased abundance in Steamed sweet potato group (SDF-S)
| NCBI | Quality Control | Links |
|---|---|---|
| Anaerotignum | ||
| Clostridium | ||
| Intestinimonas | ||
| Mucispirillum | ||
| Oscillibacter | ||
| unclassified Bacillota | ||
| unclassified Lachnospiraceae | ||
| unclassified Oscillospiraceae |
Experiment 2
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Oat Beta-glucan group (OB)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Male ICR mice administered Oat Beta-glucan group (OB) the corresponding soluble fibers at doses of 400 mg/kg bw/d
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Source: Figures 4A,B,C,K
Description: Relative Abundance of genera in the gut microbiota of mice
Abundance in Group 1: increased abundance in Oat Beta-glucan group (OB)
| NCBI | Quality Control | Links |
|---|---|---|
| unclassified Lachnospiraceae | ||
| Alistipes | ||
| unclassified Desulfovibrionaceae | ||
| Mucispirillum |
Revision editor(s): Miss Lulu
Experiment 3
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Normal control group (NC)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Normal control Male ICR mice administered saline.
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Source: Figures 4B,D,F,G,J-L
Description: Relative Abundance of genera in the gut microbiota of mice
Abundance in Group 1: increased abundance in Normal control group (NC)
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Oscillibacter | ||
| Ruminococcus | ||
| unclassified Oscillospiraceae | ||
| Mucispirillum | ||
| Intestinimonas |
Revision editor(s): Miss Lulu
Experiment 4
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Positive treatment group (PT)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Male ICR mice administered to positive treatments, the corresponding soluble fibers at doses of 400 mg/kg bw/d
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Source: Figures 4C,D,F,G,J
Description: Relative Abundance of genera in the gut microbiota of mice
Abundance in Group 1: increased abundance in Positive treatment group (PT)
| NCBI | Quality Control | Links |
|---|---|---|
| unclassified Desulfovibrionaceae | ||
| Oscillibacter | ||
| Ruminococcus | ||
| unclassified Oscillospiraceae |
Revision editor(s): Miss Lulu
Experiment 5
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Polydextrose group (PD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Male ICR mice administered to Polydextrose group (PD), the corresponding soluble fibers at doses of 400 mg/kg bw/d
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Source: Figures 4B,C,D,E,F,G,J-L
Description: Relative Abundance of genera in the gut microbiota of mice
Abundance in Group 1: increased abundance in Polydextrose group (PD)
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Mucispirillum | ||
| Oscillibacter | ||
| Ruminococcus | ||
| unclassified Bacillota | ||
| unclassified Desulfovibrionaceae | ||
| unclassified Oscillospiraceae |
Revision editor(s): Miss Lulu
Experiment 6
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Arabinogalactan group (AG)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Male ICR mice administered to Arabinogalactan group (AG), the corresponding soluble fibers at doses of 400 mg/kg bw/d
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Source: Figures 4C,D,E,G,J-L
Description: Relative Abundance of genera in the gut microbiota of mice
Abundance in Group 1: increased abundance in Arabinogalactan group (AG)
| NCBI | Quality Control | Links |
|---|---|---|
| Intestinimonas | ||
| Mucispirillum | ||
| Oscillibacter | ||
| unclassified Bacillota | ||
| unclassified Desulfovibrionaceae | ||
| unclassified Oscillospiraceae |
Revision editor(s): Miss Lulu
Experiment 7
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Inulin group (IN)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Male ICR mice administered to Inulin group (IN), the corresponding soluble fibers at doses of 400 mg/kg bw/d.
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
Source: 4D,F,G,K,L
Description: Relative Abundance of genera in the gut microbiota of mice
Abundance in Group 1: increased abundance in Inulin group (IN)
| NCBI | Quality Control | Links |
|---|---|---|
| Oscillibacter | ||
| Ruminococcus | ||
| unclassified Oscillospiraceae | ||
| Mucispirillum | ||
| Intestinimonas |
Revision editor(s): Miss Lulu
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