Probiotic supplementation mitigates sex-dependent nociceptive changes and gut dysbiosis induced by prenatal opioid exposure/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by Folakunmi on 2025-4-10
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Male offspring prenatally exposed to saline group (CSAL_M)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Male offspring prenatally exposed to opioids group (MSAL_M)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Offspring born to dams that were exposed to opioids (hydromorphone pre-gestationally followed by methadone during gestation), representing the prenatal opioid exposure condition
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 10
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
- Matched on Factors on which subjects have been matched on in a case-control study
- age
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2025-4-10
Source: Figure 3C
Description: Microbiome analysis of male offspring (CSAL_M vs. MSAL_M) (n = 10 per group in all experiments).
Abundance in Group 1: increased abundance in Male offspring prenatally exposed to opioids group (MSAL_M)
| NCBI | Quality Control | Links |
|---|---|---|
| Alloprevotella | ||
| Muribaculum | ||
| Odoribacter | ||
| Parvibacter | ||
| Tyzzerella | ||
| Lachnospiraceae bacterium NK4A136 | ||
| UGC-009UGC-009 | ||
| UGC-005UGC-005 | ||
| Prevotellaceae-Ga6A1Prevotellaceae-Ga6A1 |
Revision editor(s): Miss Lulu
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2025-4-10
Source: Figure 3C
Description: Microbiome analysis of male offspring (CSAL_M vs. MSAL_M) (n = 10 per group in all experiments).
Abundance in Group 1: decreased abundance in Male offspring prenatally exposed to opioids group (MSAL_M)
| NCBI | Quality Control | Links |
|---|---|---|
| Akkermansia | ||
| Eubacterium ventriosum | ||
| Staphylococcus |
Revision editor(s): Miss Lulu
