Gut microbiome changes with micronutrient supplementation in children with attention-deficit/hyperactivity disorder: the MADDY study/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-4-14

Curated date: 2025/03/22

Curator: Mautin

Revision editor(s): Mautin, Ese

Subjects

Location of subjects
Canada
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Micronutrient Micronutrient,micronutrient
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Placebo
Group 1 name Corresponds to the case (exposed) group for case-control studies
Micronutrient
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Children with ADHD who received micronutrient supplementation after the 8 week randomized controlled trial(RCT) period
Group 0 sample size Number of subjects in the control (unexposed) group
11
Group 1 sample size Number of subjects in the case (exposed) group
33

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Pielou Quantifies how equal the community is numerically
increased
Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-14

Curated date: 2025/03/23

Curator: Mautin

Revision editor(s): Mautin, Ese

Source: Figure 3B

Description: Changes in relative abundance in gut microbial composition following micronutrient supplementation compared to placebo

Abundance in Group 1: increased abundance in Micronutrient

NCBI Quality ControlLinks
Verrucomicrobiota

Revision editor(s): Mautin, Ese

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-4-14

Curated date: 2025/03/23

Curator: Mautin

Revision editor(s): Mautin, Ese

Source: Figure 3B

Description: Changes in relative abundance in gut microbial composition following micronutrient supplementation compared to placebo

Abundance in Group 1: decreased abundance in Micronutrient

NCBI Quality ControlLinks
Actinomycetota
Bifidobacteriaceae
Bifidobacterium
Faecalibacterium

Revision editor(s): Mautin, Ese