Newly identified species from the dog dental plaque microbiome highlight little overlap with humans

Study information

Needs review

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

39966419

DOI Digital object identifier for electronic documents.

10.1038/s41522-025-00665-2

URI Uniform resource identifier for web resources.

Authors

Heidrich V, Fackelmann G, Malesevic M, Armanini F, Dey H, Mengoni C, Stanisavljevic N, Vukotic G, Segata N

Journal

NPJ biofilms and microbiomes

Year

2025

Understudied pet-associated microbiomes represent a rich source for the discovery of microbial taxa important for pet and human health. From a cohort of 23 dogs, we sampled and metagenomically sequenced 64 dental plaque microbiomes, generating 1945 metagenome-assembled genomes spanning 347 microbial species, including 277 undercharacterized species without cultivated representatives. Integration with human microbiome data revealed the dog plaque microbiome is more diverse than - and shows little overlap (5.9% species in common) with - the human plaque microbiome, even though some shared periodontal pathobionts arise as a potential concern.

Experiment 1

Edit History Delete

Mark reviewed

Your review change was submittedDuplicate this Experiment Add a new Signature

Needs review

Curated date: 2025/06/09

Curator: Ese

Revision editor(s): Ese

Subjects

Location of subjects: Serbia

Host species Species from which microbiome was sampled. Contact us to have more species added.: Canis lupus familiaris

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Oral cavity Bucca,Buccal cavity,Cavity of mouth,Oral cavity,oral cavity

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Species design species_design,Species design,species design

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Supragingival dental plaque from humans

Group 1 name Corresponds to the case (exposed) group for case-control studies: Supragingival dental plaque from dogs

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Clinically healthy dogs with no signs of oral disease (e.g., gingivitis, periodontitis) after oral health assessment was performed at the Faculty of Veterinary Medicine, University of Belgrade

Group 0 sample size Number of subjects in the control (unexposed) group: 154

Group 1 sample size Number of subjects in the case (exposed) group: 64

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 4 weeks

Lab analysis

Sequencing type: WMS

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Signature 1

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2025/07/14

Curator: Ese

Revision editor(s): Ese

Source: Text, Paragraph 8

Description: Differentially abundant species found in Supragingival dental plaque of dogs and humans

Abundance in Group 1: increased abundance in Supragingival dental plaque from dogs

NCBI	Quality Control	Links
Campylobacter rectus

Revision editor(s): Ese

Signature 2

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2025/07/14

Curator: Ese

Revision editor(s): Ese

Source: Text, Paragraph 8

Description: Differentially abundant species found in Supragingival dental plaque of dogs and humans

Abundance in Group 1: decreased abundance in Supragingival dental plaque from dogs

NCBI	Quality Control	Links
Fusobacterium periodonticum

Revision editor(s): Ese