Roseburia hominis improves host metabolism in diet-induced obesity

From BugSigDB
Needs review
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Huang W, Zhu W, Lin Y, Chan FKL, Xu Z, Ng SC
Journal
Gut microbes
Year
2025
Keywords:
Intestinal microbiology, Obesity, probiotics
Next-generation live biotherapeutics are promising to aid the treatment of obesity and metabolic diseases. Here, we reported a novel anti-obesity probiotic candidate, Roseburia hominis, that was depleted in stool samples of obese subjects compared with lean controls, and its abundance was negatively correlated with body mass index and serum triglycerides. Supplementation of R. hominis prevented body weight gain and disorders of glucose and lipid metabolism, prevented fatty liver, inhibited white adipose tissue expansion and brown adipose tissue whitening in mice fed with high-fat diet, and boosted the abundance of lean-related species. The effects of R. hominis could be partially attributed to the production of nicotinamide riboside and upregulation of the Sirtuin1/mTOR signaling pathway. These results indicated that R. hominis is a promising candidate for the development of next-generation live biotherapeutics for the prevention of obesity and metabolic diseases.

Experiment 1


Needs review

Curated date: 2025/03/30

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Obesity , High fat diet Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity,HF - High fat diet,High fat diet (finding),High fat diet,high fat diet
Group 0 name Corresponds to the control (unexposed) group for case-control studies
High Fat Diet-Phosphate-buffered saline (HFD-PBS)
Group 1 name Corresponds to the case (exposed) group for case-control studies
High Fat Diet- Roseburia hominis (HFD-RH))
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice were fed high-fat diet (40% fat), and orally challenged with 10⁹ colony forming units of R. hominis
Group 0 sample size Number of subjects in the control (unexposed) group
5
Group 1 sample size Number of subjects in the case (exposed) group
5

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Needs review

Curated date: 2025/04/04

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4c and Figure 4d

Description: LDA effect size at the genus and species level between HFD-RH and HFD-PBS group by LEfSe

Abundance in Group 1: increased abundance in High Fat Diet- Roseburia hominis (HFD-RH))

NCBI Quality ControlLinks
Butyrivibrio
Dysosmobacter
Dysosmobacter welbionis
Faecalibacterium
Faecalibacterium prausnitzii
Flavonifractor
Flavonifractor plautii
Herbinix
Herbinix luporum
Lachnoclostridium
Lachnoclostridium sp. YL32
Lactobacillus
Lactobacillus johnsonii
Mammaliicoccus
Mammaliicoccus lentus
Oscillibacter
Oscillibacter sp. PEA192
Oscillibacter valericigenes
Parabacteroides
Parabacteroides goldsteinii
Pseudomonas
Roseburia
Roseburia intestinalis
Ruthenibacterium
Ruthenibacterium lactatiformans
Thomasclavelia
Turicimonas
Turicimonas muris
Oscillibacter sp. NSJ 62Oscillibacter sp. NSJ 62
[Clostridium] innocuum
Butyrivibrio fibrisolvens

Revision editor(s): Z.uk.a

Signature 2

Needs review

Curated date: 2025/04/04

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4c and Figure 4d

Description: LDA effect size at the genus and species level between HFD-RH and HFD-PBS group by LEfSe

Abundance in Group 1: decreased abundance in High Fat Diet- Roseburia hominis (HFD-RH))

NCBI Quality ControlLinks
Eggerthella
Faecalibaculum
Faecalibaculum rodentium
Olsenella
Olsenella sp. GAM18
Olsenella sp. oral taxon 807
Olsenella uli
Parafannyhessea umbonata
Parolsenella
Parolsenella catena
Parolsenella massiliensis
Romboutsia
Staphylococcus
Staphylococcus xylosus
Thermophilibacter immobilis
eggerthella sp. HF 1101eggerthella sp. HF 1101
Berryella intestinalis

Revision editor(s): Z.uk.a

Experiment 2


Needs review

Curated date: 2025/04/10

Curator: Z.uk.a

Revision editor(s): Z.uk.a, Victoria

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Body weight gain Body weight gain,body weight gain
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Low Body Weight
Group 1 name Corresponds to the case (exposed) group for case-control studies
High Body Weight
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
The Mice were characterized based on body weight gain.

Lab analysis

Statistical Analysis

Statistical test
Spearman Correlation
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
Not specified


Signature 1

Needs review

Curated date: 2025/04/10

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Body weight gain correlation differential species in mice between HFD-PBS and HFD-RH groups

Abundance in Group 1: increased abundance in High Body Weight

NCBI Quality ControlLinks
Romboutsia ilealis
Staphylococcus xylosus

Revision editor(s): Z.uk.a

Signature 2

Needs review

Curated date: 2025/04/10

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Body weight gain correlation of differential species in mice between HFD-PBS and HFD-RH groups

Abundance in Group 1: decreased abundance in High Body Weight

NCBI Quality ControlLinks
Herbinix luporum
Turicimonas muris
Butyrivibrio fibrisolvens

Revision editor(s): Z.uk.a

Experiment 3


Needs review

Curated date: 2025/04/10

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Body mass index BMI,Quetelet's Index,Body mass index,body mass index
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Low Body Mass Index
Group 1 name Corresponds to the case (exposed) group for case-control studies
High Body Mass Index
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
The Mice were differentiated based on changes in Body Mass Index.

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2025/04/11

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Body Mass Index correlation of differential species in mice between HFD-PBS and HFD-RH groups

Abundance in Group 1: increased abundance in High Body Mass Index

NCBI Quality ControlLinks
Romboutsia ilealis
Staphylococcus xylosus
Faecalibaculum rodentium

Revision editor(s): Z.uk.a

Signature 2

Needs review

Curated date: 2025/04/11

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Body Mass Index correlation of differential species in mice between HFD-PBS and HFD-RH groups

Abundance in Group 1: decreased abundance in High Body Mass Index

NCBI Quality ControlLinks
Dysosmobacter welbionis
Faecalibacterium prausnitzii
Herbinix luporum
Lachnoclostridium sp. YL32
Oscillibacter sp. PEA192
Oscillibacter valericigenes
Parabacteroides goldsteinii
Oscillibacter sp. NSJ 62Oscillibacter sp. NSJ 62
Butyrivibrio fibrisolvens

Revision editor(s): Z.uk.a

Experiment 4


Needs review

Curated date: 2025/04/11

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Body fat percentage Body fat percentage,body fat percentage
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Low Body Fat Percentage [Lee Index}
Group 1 name Corresponds to the case (exposed) group for case-control studies
High Body Fat Percentage [Lee Index]
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Group 1 consists of mice who were characterized by their Body fat percentage (Lee Index)

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2025/04/11

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Body Fat Percentage correlation differential species in mice between HFD-PBS and HFD-RH groups

Abundance in Group 1: increased abundance in High Body Fat Percentage [Lee Index]

NCBI Quality ControlLinks
Romboutsia ilealis
Staphylococcus xylosus

Revision editor(s): Z.uk.a

Signature 2

Needs review

Curated date: 2025/04/11

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Body Fat Percentage correlation differential species in mice between HFD-PBS and HFD-RH groups

Abundance in Group 1: decreased abundance in High Body Fat Percentage [Lee Index]

NCBI Quality ControlLinks
Dysosmobacter welbionis
Faecalibacterium prausnitzii
Lachnoclostridium sp. YL32
Lactobacillus johnsonii
Oscillibacter valericigenes
Parabacteroides goldsteinii
Turicimonas muris

Revision editor(s): Z.uk.a

Experiment 5


Needs review

Curated date: 2025/04/11

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Insulin receptor measurement Insulin receptor measurement,insulin receptor measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Presence of Insulin Receptor
Group 1 name Corresponds to the case (exposed) group for case-control studies
Absence of Insulin Receptor
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
The mice in Group 1 were selected as those with no insulin receptors.

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2025/04/13

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Insulin Receptors correlation differential species in mice between HFD-PBS and HFD-RH groups

Abundance in Group 1: increased abundance in Absence of Insulin Receptor

NCBI Quality ControlLinks
Romboutsia ilealis
Staphylococcus xylosus

Revision editor(s): Z.uk.a

Signature 2

Needs review

Curated date: 2025/04/13

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Insulin receptors correlation differential species in mice between HFD-PBS and HFD-RH groups

Abundance in Group 1: decreased abundance in Absence of Insulin Receptor

NCBI Quality ControlLinks
Lachnoclostridium sp. YL32
Lactobacillus johnsonii
Parabacteroides goldsteinii
Turicimonas muris

Revision editor(s): Z.uk.a

Experiment 6


Needs review

Curated date: 2025/04/11

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Total cholesterol measurement cholesterol, total,total cholesterol levels,Total cholesterol measurement,total cholesterol measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Low total cholesterol
Group 1 name Corresponds to the case (exposed) group for case-control studies
High total cholesterol
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Group 1 consists of mice with High Cholesterol levels.

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2025/04/13

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Total Cholesterol correlation of differential species in mice between HFD-PBS and HFD-RH groups

Abundance in Group 1: increased abundance in High total cholesterol

NCBI Quality ControlLinks
Faecalibaculum rodentium
Romboutsia ilealis
Staphylococcus xylosus
Eggerthella sp. HF 1101Eggerthella sp. HF 1101
Berryella intestinalis

Revision editor(s): Z.uk.a

Signature 2

Needs review

Curated date: 2025/04/13

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Total Cholesterol correlation differential species in mice between HFD-PBS and HFD-RH groups

Abundance in Group 1: decreased abundance in High total cholesterol

NCBI Quality ControlLinks
Dysosmobacter welbionis
Faecalibacterium prausnitzii
Flavonifractor plautii
Herbinix luporum
Lachnoclostridium sp. YL32
Oscillibacter sp. PEA192
Oscillibacter valericigenes
Parabacteroides goldsteinii
Roseburia hominis
Roseburia intestinalis
Ruthenibacterium lactatiformans
Oscillibacter sp. NSJ 62Oscillibacter sp. NSJ 62
Butyrivibrio fibrisolvens

Revision editor(s): Z.uk.a

Experiment 7


Needs review

Curated date: 2025/04/11

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Triglyceride change measurement Triglyceride change measurement,triglyceride change measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Low triglycerides
Group 1 name Corresponds to the case (exposed) group for case-control studies
High triglycerides
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Group 1 Mice were distinguished by High Triglyceride levels

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2025/04/13

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Triglyceride correlation differential species in mice between HFD-PBS and HFD-RH groups

Abundance in Group 1: increased abundance in High triglycerides

NCBI Quality ControlLinks
Faecalibaculum rodentium
Staphylococcus xylosus

Revision editor(s): Z.uk.a

Signature 2

Needs review

Curated date: 2025/04/14

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Triglyceride correlation of differential species in mice between HFD-PBS and HFD-RH groups

Abundance in Group 1: decreased abundance in High triglycerides

NCBI Quality ControlLinks
Dysosmobacter welbionis
Faecalibacterium prausnitzii
Herbinix luporum
Lachnoclostridium sp. YL32
Lactobacillus johnsonii
Oscillibacter valericigenes
Roseburia hominis
Roseburia intestinalis
Turicimonas muris
[Clostridium] innocuum
Butyrivibrio fibrisolvens

Revision editor(s): Z.uk.a

Experiment 8


Needs review

Curated date: 2025/04/11

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Aspartate aminotransferase measurement Aspartate transaminase measurement,Aspartate aminotransferase measurement,aspartate aminotransferase measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
High in aspartate aminotransferase
Group 1 name Corresponds to the case (exposed) group for case-control studies
Low in aspartate aminotransferase
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Group 1 consists of mice with low levels of aspartate aminotransferase enzymes

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2025/04/14

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Aspartate aminotransferase levels show differential correlation between species in mice from the HFD-PBS and HFD-RH groups

Abundance in Group 1: increased abundance in Low in aspartate aminotransferase

NCBI Quality ControlLinks
Faecalibaculum rodentium
Romboutsia ilealis
Staphylococcus xylosus

Revision editor(s): Z.uk.a

Signature 2

Needs review

Curated date: 2025/04/14

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Aspartate aminotransferase levels show differential correlation between species in mice from the HFD-PBS and HFD-RH groups

Abundance in Group 1: decreased abundance in Low in aspartate aminotransferase

NCBI Quality ControlLinks
Lachnoclostridium sp. YL32
Lactobacillus johnsonii
Parabacteroides goldsteinii

Revision editor(s): Z.uk.a

Experiment 9


Needs review

Curated date: 2025/04/11

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Alanine measurement Alanine measurement,alanine measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
High in alanine transaminase
Group 1 name Corresponds to the case (exposed) group for case-control studies
Low in alanine transaminase
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice in this group are characterized by low levels of alanine transaminase enzymes

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2025/04/14

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Alanine transaminase correlation of differential species in mice between the HFD-PBS and HFD-RH groups

Abundance in Group 1: increased abundance in Low in alanine transaminase

NCBI Quality ControlLinks
Faecalibaculum rodentium

Revision editor(s): Z.uk.a

Signature 2

Needs review

Curated date: 2025/04/14

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Alanine transaminase correlation of differential species in mice between the HFD-PBS and HFD-RH groups

Abundance in Group 1: decreased abundance in Low in alanine transaminase

NCBI Quality ControlLinks
Herbinix luporum
Lachnoclostridium sp. YL32
Butyrivibrio fibrisolvens
[Clostridium] innocuum
Lactobacillus johnsonii

Revision editor(s): Z.uk.a

Experiment 11


Needs review

Curated date: 2025/04/11

Curator: Z.uk.a

Revision editor(s): Z.uk.a, Victoria

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Triglyceride C6H5O6R3,Triacylglycerol,triacylglycerols,Triglycerid,Triglyceride,triglycerides,Triglyzerid,triglyceride
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Low Hepatic triglycerides
Group 1 name Corresponds to the case (exposed) group for case-control studies
High Hepatic triglycerides
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
The mice in group 1 consists of mice with High levels of hepatic triglycerides

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2025/04/14

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Hepatic triglyceride correlation of differential species in mice between the HFD-PBS and HFD-RH groups

Abundance in Group 1: increased abundance in High Hepatic triglycerides

NCBI Quality ControlLinks
Faecalibaculum rodentium
Romboutsia ilealis
Staphylococcus xylosus
Eggerthella sp. HF 1101Eggerthella sp. HF 1101
Berryella intestinalis

Revision editor(s): Z.uk.a

Signature 2

Needs review

Curated date: 2025/04/14

Curator: Z.uk.a

Revision editor(s): Z.uk.a

Source: Figure 4E

Description: Hepatic triglyceride levels exhibit differential correlations with species in mice between the HFD-PBS and HFD-RH groups

Abundance in Group 1: decreased abundance in High Hepatic triglycerides

NCBI Quality ControlLinks
Dysosmobacter welbionis
Faecalibacterium prausnitzii
Lactobacillus johnsonii
Oscillibacter sp. PEA192
Oscillibacter valericigenes
Parabacteroides goldsteinii
Turicimonas muris

Revision editor(s): Z.uk.a