Increased dietary protein stimulates amino acid catabolism via the gut microbiota and secondary bile acid production/Experiment 5
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2025-4-4
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Colon Hindgut,Large bowel,Posterior intestine,Colon,colon
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to diet Response to diet,response to diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Low protein group- LP
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Normal protein group- NP
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Dietary regimen constituting of 20% protein for 10 days.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 20
- Group 1 sample size Number of subjects in the case (exposed) group
- 20
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- ANOVA
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-4-4
Source: Figure 4D
Description: Gut microbiota is modified by dietary protein concentration in mice (genus)
Abundance in Group 1: increased abundance in Normal protein group- NP
| NCBI | Quality Control | Links |
|---|---|---|
| AF12AF12 | ||
| Coprococcus | ||
| Oscillospira | ||
| Sutterella | ||
| Lactobacillus |
Revision editor(s): Miss Lulu
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-4-4
Source: Figure 4D
Description: Gut microbiota is modified by dietary protein concentration in mice.(genus)
Abundance in Group 1: decreased abundance in Normal protein group- NP
| NCBI | Quality Control | Links |
|---|---|---|
| Rikenella | ||
| SMB53SMB53 | ||
| Allobaculum |
Revision editor(s): Miss Lulu
