Gut microbial dysbiosis exacerbates long-term cognitive impairments by promoting intestinal dysfunction and neuroinflammation following neonatal hypoxia-ischemia/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-4-22

Curated date: 2025/03/20

Curator: IsaacImitini

Revision editor(s): IsaacImitini, Tosin

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Rattus norvegicus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Brain hypoxia-Ischemia Hypoxia-Ischemia, Brain,Brain hypoxia-Ischemia,brain hypoxia-Ischemia
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Sham Group
Group 1 name Corresponds to the case (exposed) group for case-control studies
HI (hypoxic-ischemic) Group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Neonatal rats that underwent hypoxia-ischema (HI) to induce brain injury
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-22

Curated date: 2025/03/26

Curator: IsaacImitini

Revision editor(s): IsaacImitini, Tosin

Source: Figure 2I and 2J

Description: Taxonomic significant differences in gut microbiota between rats in sham group and HI (hypoxic-ischemic) group using Lefse analysis

Abundance in Group 1: increased abundance in HI (hypoxic-ischemic) Group

NCBI Quality ControlLinks
Deferribacteraceae
Deferribacterales
Deferribacteres
Enterobacterales
Enterobacteriaceae
Fusobacteriaceae
Fusobacteriales
Fusobacteriia
Gammaproteobacteria
Lactobacillaceae
Prevotellaceae
Streptococcaceae
Vibrionaceae

Revision editor(s): IsaacImitini, Tosin

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-4-22

Curated date: 2025/03/27

Curator: IsaacImitini

Revision editor(s): IsaacImitini, Tosin

Source: Figure 2I and 2J

Description: Taxonomic significant differences in gut microbiota between rats in sham group and HI (hypoxic-ischemic) group using LeFse analysis

Abundance in Group 1: decreased abundance in HI (hypoxic-ischemic) Group

NCBI Quality ControlLinks
Akkermansiaceae
Bacteroidaceae
Enterococcaceae
Terriglobia
Verrucomicrobiales
Verrucomicrobiia
Victivallaceae
Helicobacteraceae
Caryophanaceae

Revision editor(s): IsaacImitini, Tosin