Therapeutic Mechanism of Zhuyang Tongbian Decoction in Treating Functional Constipation: Insights from a Pilot Study Utilizing 16S rRNA Sequencing, Metagenomics, and Metabolomics/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-4-7

Curated date: 2025/03/21

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
NC (Healthy Volunteers)
Group 1 name Corresponds to the case (exposed) group for case-control studies
FC_PreZY (Functional Constipation in Pre-Zhuyang Tongbian Decoction) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
FC_PreZY group was treated with Zhuyang Tongbian Decoction (ZTD) (raw drug concentration: 3.0 g/mL)
Group 0 sample size Number of subjects in the control (unexposed) group
20
Group 1 sample size Number of subjects in the case (exposed) group
20
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
decreased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-7

Curated date: 2025/03/21

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: Fig 2c

Description: Comparison of significantly different flora before and after treatment with ZTD at the genus level

Abundance in Group 1: increased abundance in FC_PreZY (Functional Constipation in Pre-Zhuyang Tongbian Decoction) group

NCBI Quality ControlLinks
Collinsella
Desulfovibrio
Romboutsia
Ruminococcus
UCG-002UCG-002
rumen bacterium NK4A214

Revision editor(s): PreciousChijioke

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-4-7

Curated date: 2025/03/21

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: Fig 2c

Description: Comparison of significantly different flora before and after treatment with ZTD at the genus level

Abundance in Group 1: decreased abundance in FC_PreZY (Functional Constipation in Pre-Zhuyang Tongbian Decoction) group

NCBI Quality ControlLinks
Agathobacter
Faecalibacterium
Fusicatenibacter
Subdoligranulum
Christensenellaceae_R_7_groupChristensenellaceae_R_7_group

Revision editor(s): PreciousChijioke