Therapeutic Mechanism of Zhuyang Tongbian Decoction in Treating Functional Constipation: Insights from a Pilot Study Utilizing 16S rRNA Sequencing, Metagenomics, and Metabolomics/Experiment 5

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-4-7

Curated date: 2025/03/22

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, KateRasheed

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
NC (Healthy volunteers) + FC_PreZY (Functional Constipation in Pre-Zhuyang Tongbian Decoction)
Group 1 name Corresponds to the case (exposed) group for case-control studies
ProZY (Pro-Zhuyang Tongbian Decoction)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
FC_PreZY group after treatment with ZTD (raw drug concentration: 3.0 g/mL)
Group 0 sample size Number of subjects in the control (unexposed) group
20
Group 1 sample size Number of subjects in the case (exposed) group
20
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2


Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-7

Curated date: 2025/03/23

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, KateRasheed

Source: Fig 4

Description: Analysis of differential flora at different taxonomic levels

Abundance in Group 1: increased abundance in ProZY (Pro-Zhuyang Tongbian Decoction)

NCBI Quality ControlLinks
Bacteroides clarus
Butyricicoccaceae
Butyricicoccus
Christensenellaceae
Christensenellales
Lachnospira
Lactobacillales
Marinifilaceae
Oscillibacter
Paraprevotella
Streptococcaceae
Streptococcus
Streptococcus salivarius
Veillonella
Veillonella parvula
gut metagenome
Christensenellaceae_R_7_groupChristensenellaceae_R_7_group
UCG_008UCG_008
Clostridia UCG_014Clostridia UCG_014
Bacilli
Lachnospiraceae bacterium NK4A136
Lachnospira eligens
Prevotellaceae NK3B31 groupPrevotellaceae NK3B31 group
Enterococcaceae bacterium RF39
Lachnospiraceae UCG-001Lachnospiraceae UCG-001
[Eubacterium] siraeum

Revision editor(s): PreciousChijioke, KateRasheed