Seasonal variations in circulating endocannabinoidome mediators and gut microbiota composition in humans
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Castonguay-Paradis S, Demers-Potvin É, Rochefort G, Lacroix S, Perron J, Martin C, Flamand N, Raymond F, Di Marzo V, Veilleux A
Journal
Gut microbes
Year
2025
Keywords:
2‑monoacylglycerol, Dietary intakes, Gut microbiota, N-acylethanolamines, Seasons, Vitamin D
BACKGROUND: The human gut microbiome-endocannabinoidome axis is crucial for several homeostatic processes, including inflammation and energy metabolism, and is influenced by many endogenous and exogenous factors, such as dietary habits. Changes in the gut microbiome in response to seasonal variations were previously reported and tentatively attributed to shifts in dietary patterns. However, there is a need for longitudinal studies in industrialized populations to comprehensively explore seasonal variations independently of lifestyle confounding factors. OBJECTIVE: To investigate the longitudinal effects of seasonal variations on the composition of the gut microbiome and the circulating levels of endocannabinoidome mediators in humans, while elucidating the contributing factors underlying these changes. METHODS: Plasma and fecal samples were collected at the end of both the winter and summer in a longitudinal cohort of 48 individuals living in Québec City (Canada). Dietary habits, medical history, fecal microbiota taxonomic composition and plasma levels of circulating N‑acyl‑ethanolamines (NAEs) and 2‑monoacyl-glycerols (2‑MAGs) were obtained at each time point. RESULTS: Lower circulating levels of most NAEs were observed at the end of summer. These changes were accompanied by a reduction in the relative abundance of the Bifidobacteriaceae and Lachnospiraceae families, along with an increase in the abundance of the Bacteroidaceae and Ruminococcaceae families. These seasonal variations were not associated with concurrent changes in adiposity parameters, dietary intakes, physical activity habits, or vitamin D status. Importantly, the magnitude of the shift in gut microbiota composition from winter to summer was found to be associated with the seasonal variations in circulating endocannabinoidome (eCBome) mediators. CONCLUSION: This study identified specific seasonal changes in gut microbiota composition and circulating levels of several NAEs, which were not associated with vitamin D status and lifestyle habits. It underscores the importance of the gut microbiota-endocannabinoidome axis in the pathophysiology of seasonal changes, and of considering seasons in clinical trials on these systems.
Experiment 1
Reviewed Marked as Reviewed by KateRasheed on 2025-6-9
Subjects
- Location of subjects
- Canada
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Seasonality measurement Seasonality measurement,seasonality measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Winter season
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Summer season
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants who paid a visit the Institute of Nutrition and Functional Foods (INAF) in summer season
- Group 0 sample size Number of subjects in the control (unexposed) group
- 204
- Group 1 sample size Number of subjects in the case (exposed) group
- 50
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-6-9
Source: Figure 2a and 2b
Description: Cladogram representation of the paired-wise Wilcoxon analysis showing the taxonomic ranks from the innermost phylum ring to the outermost genera ring and boxplots representation of significant taxonomic ranks including the median, lower/higher quartiles and 1.5× inter-quartile range whiskers.
Abundance in Group 1: increased abundance in Summer season
Revision editor(s): Tosin
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-6-9
Source: Figure 2a and 2b
Description: Cladogram representation of the paired-wise Wilcoxon analysis showing the taxonomic ranks from the innermost phylum ring to the outermost genera ring and boxplots representation of significant taxonomic ranks including the median, lower/higher quartiles and 1.5× inter-quartile range whiskers.
Abundance in Group 1: decreased abundance in Summer season
Revision editor(s): Tosin
Experiment 2
Reviewed Marked as Reviewed by KateRasheed on 2025-6-9
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Low Seasonal Changes
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- High Seasonal Changes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants who were categorized to have been observed with higher composition of gut microbiota associated with seasonal changes in the circulating levels of NAEs (N-acyl-ethanolamines)
- Group 0 sample size Number of subjects in the control (unexposed) group
- Not specified
- Group 1 sample size Number of subjects in the case (exposed) group
- Not specified
Lab analysis
Statistical Analysis
- Statistical test
- LEfSe
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 1.5
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-6-9
Source: Figure 7D
Description: Linear discriminant analysis effect size (LEfSe) analysis identifying microbial taxa associated with seasonal changes in the dim 3 family groups and, consequently, with the circulating NAEs seasonal changes observed (Kruskal-Wallis tests p < 0.05 and LDA score >1.5)
Abundance in Group 1: increased abundance in High Seasonal Changes
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-6-9
Source: Figure 7D
Description: linear discriminant analysis effect size (LEfSe) analysis identifying microbial taxa associated with seasonal changes in the dim 3 family groups and, consequently, with the circulating NAEs seasonal changes observed (Kruskal-Wallis tests p < 0.05 and LDA score >1.5)
Abundance in Group 1: decreased abundance in High Seasonal Changes
| NCBI | Quality Control | Links |
|---|---|---|
| Acidaminococcus | ||
| Blautia | ||
| Dialister | ||
| Faecalibacterium | ||
| Lactobacillus | ||
| Ruminococcus_1Ruminococcus_1 |
Revision editor(s): Montana-D
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