Multiple sclerosis and gut microbiota: Lachnospiraceae from the ileum of MS twins trigger MS-like disease in germfree transgenic mice-An unbiased functional study/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by KateRasheed on 2025-6-24

Curated date: 2025/05/01

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Subjects

Location of subjects
Germany
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Multiple sclerosis Disseminated Sclerosis,generalized multiple sclerosis,insular sclerosis,MS,MS (Multiple Sclerosis),multiple sclerosis,MULTIPLE SCLEROSIS ACUTE FULMINATING,Multiple Sclerosis, Acute Fulminating,Sclerosis, Disseminated,Sclerosis, Multiple,Multiple sclerosis
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Unaffected twins
Group 1 name Corresponds to the case (exposed) group for case-control studies
Affected twins
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Individuals with Multiple Sclerosis, from monozygotic twin pairs clinically discordant for multiple sclerosis.
Group 0 sample size Number of subjects in the control (unexposed) group
81
Group 1 sample size Number of subjects in the case (exposed) group
81
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Chi-Square
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-6-24

Curated date: 2025/05/01

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Figure 1E

Description: A phylogenetic tree showing the taxa nominally significant in Chi-squared test. The “Trend” in the legend indicates changes in abundance in affected twins (compared to unaffected twins).

Abundance in Group 1: increased abundance in Affected twins

NCBI Quality ControlLinks
Acutalibacter
Anaerotruncus colihominis
Bacteroidales
Barnesiella intestinihominis
Candidatus Limenecus avicola
Candidatus Scatocola faecipullorum
Collinsella tanakaei
Coprobacillus cateniformis
Eisenbergiella tayi
Enorma massiliensis
Enterococcaceae
Eubacteriales
Faecalicoccus pleomorphus
Negativibacillus massiliensis
Oscillospiraceae
Paraprevotella xylaniphila
Sakamotonia
[Eubacterium] siraeum
Hungatella_A_128155Hungatella_A_128155
WRMH01 sp009777235WRMH01 sp009777235
UBA6984 sp003258725UBA6984 sp003258725
UMGS1490UMGS1490
Alistipes_A_871404 ihumiiAlistipes_A_871404 ihumii
Clostridium sp. CAG:632
Ruminococcaceae bacterium UBA5905

Revision editor(s): Anne-mariesharp

Signature 2

Reviewed Marked as Reviewed by KateRasheed on 2025-6-24

Curated date: 2025/05/01

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Figure 1E

Description: A phylogenetic tree showing the taxa nominally significant in Chi-squared test. The “Trend” in the legend indicates changes in abundance in affected twins (compared to unaffected twins).

Abundance in Group 1: decreased abundance in Affected twins

NCBI Quality ControlLinks
Achromobacter
Collinsella
Dialister succinatiphilus
Gemella
Holdemania massiliensis
Leyella stercorea
Mitsuokella jalaludinii
Romboutsia ilealis
Segatella buccae
Ruminococcus_E sp900314705Ruminococcus_E sp900314705
CAG_475 sp900548005CAG_475 sp900548005
SFM101 sp004556155SFM101 sp004556155
Caecibacter sp003467125Caecibacter sp003467125
Prevotella sp002251435Prevotella sp002251435
Comamonas_F_589250Comamonas_F_589250
SFLA01SFLA01
Clostridium neonatale

Revision editor(s): Anne-mariesharp