Multiple sclerosis and gut microbiota: Lachnospiraceae from the ileum of MS twins trigger MS-like disease in germfree transgenic mice-An unbiased functional study/Experiment 6

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-24

Curated date: 2025/05/01

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Subjects

Location of subjects
Germany
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Ileum Distal intestine,Intestinum ileum,Lower intestine,Posterior intestine,Ileum,ileum
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Transplant outcome measurement Transplant outcome measurement,transplant outcome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
GF_HT-Q (Germ Free_Healthy Twin)
Group 1 name Corresponds to the case (exposed) group for case-control studies
GF_MS-Q (Germ Free_Multiple Sclerosis)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Germ-free Gnotobiotic RR (Relapsing Remitting) SJL/J mice colonized with ileal microbiota from the Multiple Sclerosis twin (MS-Q)
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-6-24

Curated date: 2025/05/01

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig. S4G

Description: Relative abundance of ileal microbiota in humanized gnotobiotic RR mice that showed significant differences (unpaired t-test) at the genus level.

Abundance in Group 1: increased abundance in GF_MS-Q (Germ Free_Multiple Sclerosis)

NCBI Quality ControlLinks
Lachnoclostridium
Escherichia/Shigella sp.

Revision editor(s): Anne-mariesharp

Signature 2

Reviewed Marked as Reviewed by KateRasheed on 2025-6-24

Curated date: 2025/05/01

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig. S4G

Description: Relative abundance of ileal microbiota in humanized gnotobiotic RR mice that showed significant differences (unpaired t-test) at the genus level.

Abundance in Group 1: decreased abundance in GF_MS-Q (Germ Free_Multiple Sclerosis)

NCBI Quality ControlLinks
Akkermansia
Thomasclavelia
Streptococcus

Revision editor(s): Anne-mariesharp