Multiple sclerosis and gut microbiota: Lachnospiraceae from the ileum of MS twins trigger MS-like disease in germfree transgenic mice-An unbiased functional study/Experiment 6
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-6-24
Subjects
- Location of subjects
- Germany
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Ileum Distal intestine,Intestinum ileum,Lower intestine,Posterior intestine,Ileum,ileum
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Transplant outcome measurement Transplant outcome measurement,transplant outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- GF_HT-Q (Germ Free_Healthy Twin)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- GF_MS-Q (Germ Free_Multiple Sclerosis)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Germ-free Gnotobiotic RR (Relapsing Remitting) SJL/J mice colonized with ileal microbiota from the Multiple Sclerosis twin (MS-Q)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-6-24
Source: Fig. S4G
Description: Relative abundance of ileal microbiota in humanized gnotobiotic RR mice that showed significant differences (unpaired t-test) at the genus level.
Abundance in Group 1: increased abundance in GF_MS-Q (Germ Free_Multiple Sclerosis)
| NCBI | Quality Control | Links |
|---|---|---|
| Lachnoclostridium | ||
| Escherichia/Shigella sp. |
Revision editor(s): Anne-mariesharp
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-6-24
Source: Fig. S4G
Description: Relative abundance of ileal microbiota in humanized gnotobiotic RR mice that showed significant differences (unpaired t-test) at the genus level.
Abundance in Group 1: decreased abundance in GF_MS-Q (Germ Free_Multiple Sclerosis)
| NCBI | Quality Control | Links |
|---|---|---|
| Akkermansia | ||
| Thomasclavelia | ||
| Streptococcus |
Revision editor(s): Anne-mariesharp
