Multiple sclerosis and gut microbiota: Lachnospiraceae from the ileum of MS twins trigger MS-like disease in germfree transgenic mice-An unbiased functional study/Experiment 8
From BugSigDB
Needs review
Subjects
- Location of subjects
- Germany
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Transplant outcome measurement Transplant outcome measurement,transplant outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- GF_HT (Germ Free_Healthy Twin) (End)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- GF_MS (Germ Free_Multiple Sclerosis) (End)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Germ-free Gnotobiotic RR (Relapsing Remitting) SJL/J mice colonized with ileal microbiota from the Multiple sclerosis twin (MS-A) (at endpoint after colonization)
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Needs review
Source: Fig S5
Description: Relative abundance of fecal microbiota from humanized gnotobiotic RR mice that showed significant changes (unpaired t-test) at the genus level 2 weeks after the colonization and at the endpoint.
Abundance in Group 1: increased abundance in GF_MS (Germ Free_Multiple Sclerosis) (End)
| NCBI | Quality Control | Links |
|---|---|---|
| Anaerostipes | ||
| Clostridium | ||
| Flavonifractor | ||
| Lachnoclostridium | ||
| [Clostridium] innocuum |
Revision editor(s): Anne-mariesharp
Signature 2
Needs review
Source: Fig S5
Description: Relative abundance of fecal microbiota from humanized gnotobiotic RR mice that showed significant changes (unpaired t-test) at the genus level 2 weeks after the colonization and at the endpoint.
Abundance in Group 1: decreased abundance in GF_MS (Germ Free_Multiple Sclerosis) (End)
| NCBI | Quality Control | Links |
|---|---|---|
| Staphylococcus |
Revision editor(s): Anne-mariesharp
