Multiple sclerosis and gut microbiota: Lachnospiraceae from the ileum of MS twins trigger MS-like disease in germfree transgenic mice-An unbiased functional study/Experiment 8

From BugSigDB


Needs review

Curated date: 2025/05/01

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Subjects

Location of subjects
Germany
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Transplant outcome measurement Transplant outcome measurement,transplant outcome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
GF_HT (Germ Free_Healthy Twin) (End)
Group 1 name Corresponds to the case (exposed) group for case-control studies
GF_MS (Germ Free_Multiple Sclerosis) (End)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Germ-free Gnotobiotic RR (Relapsing Remitting) SJL/J mice colonized with ileal microbiota from the Multiple sclerosis twin (MS-A) (at endpoint after colonization)

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Signature 1

Needs review

Curated date: 2025/05/01

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig S5

Description: Relative abundance of fecal microbiota from humanized gnotobiotic RR mice that showed significant changes (unpaired t-test) at the genus level 2 weeks after the colonization and at the endpoint.

Abundance in Group 1: increased abundance in GF_MS (Germ Free_Multiple Sclerosis) (End)

NCBI Quality ControlLinks
Anaerostipes
Clostridium
Flavonifractor
Lachnoclostridium
[Clostridium] innocuum

Revision editor(s): Anne-mariesharp

Signature 2

Needs review

Curated date: 2025/05/01

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig S5

Description: Relative abundance of fecal microbiota from humanized gnotobiotic RR mice that showed significant changes (unpaired t-test) at the genus level 2 weeks after the colonization and at the endpoint.

Abundance in Group 1: decreased abundance in GF_MS (Germ Free_Multiple Sclerosis) (End)

NCBI Quality ControlLinks
Staphylococcus

Revision editor(s): Anne-mariesharp