Initial gut microbiota composition is a determining factor in the promotion of colorectal cancer by oral iron supplementation: evidence from a murine model/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by KateRasheed on 2025-6-9

Curated date: 2025/04/25

Curator: Joiejoie

Revision editor(s): Joiejoie, PreciousChijioke

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Transplant outcome measurement Transplant outcome measurement,transplant outcome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
FMT-HC (Fecal microbiota transplantation from healthy controls) mice fed 50 ppm iron
Group 1 name Corresponds to the case (exposed) group for case-control studies
FMT-HC (Fecal microbiota transplantation from healthy controls) mice fed 500 ppm iron
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
This group refers to mice that received fecal microbiota transplantation from healthy human controls and were fed an iron excess (500 ppm) diet.
Group 0 sample size Number of subjects in the control (unexposed) group
24
Group 1 sample size Number of subjects in the case (exposed) group
26

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V5-V6
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
unchanged

Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-6-9

Curated date: 2025/04/25

Curator: Joiejoie

Revision editor(s): Joiejoie, PreciousChijioke

Source: Fig. 2B, 2E and S7

Description: Relative abundances of microbial taxa at phylum level.

Abundance in Group 1: increased abundance in FMT-HC (Fecal microbiota transplantation from healthy controls) mice fed 500 ppm iron

NCBI Quality ControlLinks
Bacteroidota
Pseudomonadota
Verrucomicrobiota
Alistipes inops
Romboutsia ilealis

Revision editor(s): Joiejoie, PreciousChijioke

Signature 2

Reviewed Marked as Reviewed by KateRasheed on 2025-6-9

Curated date: 2025/04/25

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Victoria

Source: Fig. 2B, 2E and S7

Description: Relative abundances of microbial taxa at phylum level.

Abundance in Group 1: decreased abundance in FMT-HC (Fecal microbiota transplantation from healthy controls) mice fed 500 ppm iron

NCBI Quality ControlLinks
Actinomycetota
Bacillota
Bifidobacterium pseudolongum

Revision editor(s): PreciousChijioke, Victoria