Initial gut microbiota composition is a determining factor in the promotion of colorectal cancer by oral iron supplementation: evidence from a murine model/Experiment 8

From BugSigDB


Reviewed Marked as Reviewed by KateRasheed on 2025-6-9

Curated date: 2025/05/13

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Victoria

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Diet measurement Diet measurement,diet measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Mice fed with Saline 50ppm
Group 1 name Corresponds to the case (exposed) group for case-control studies
Mice fed with Saline 500ppm
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
This group refers to mice that received fecal microbiota transplantation from Colorectal cancer patients and were fed with a Saline (500 ppm) diet.

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V5-V6
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
ANOVA
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes


Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-6-9

Curated date: 2025/05/13

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Victoria

Source: Fig. 5B

Description: Fecal levels of A. inops (Ai) and B. pseudolongum (Bp) quantified by real-time PCR in mice at endpoint.

Abundance in Group 1: increased abundance in Mice fed with Saline 500ppm

NCBI Quality ControlLinks
Alistipes inops

Revision editor(s): PreciousChijioke, Victoria

Signature 2

Reviewed Marked as Reviewed by KateRasheed on 2025-6-9

Curated date: 2025/06/05

Curator: Victoria

Revision editor(s): Victoria

Source: Figure 5B

Description: Fecal levels of A. inops (Ai) and B. pseudolongum (Bp) quantified by real-time PCR in mice at endpoint.

Abundance in Group 1: decreased abundance in Mice fed with Saline 500ppm

NCBI Quality ControlLinks
Bifidobacterium pseudolongum

Revision editor(s): Victoria