Background: Ulcerative colitis (Uc), which is characterized by chronic relapsing inflammation ofthe colon, results from a complex interaction of factors involving the host, environment, andmicrobiome. the present study aimed to investigate the gut microbial composition and metabolicvariations in patients with Uc and their spouses. Materials and Methods: Fecal samples werecollected from 13 healthy spouses and couples with Uc. 16s rRNa gene amplicon sequencingand metagenomics sequencing were used to analyze gut microbiota composition, pathways,gene expression, and enzyme activity, followed by the Kyoto encyclopedia of Genes and Genomes.Results: We found that the microbiome diversity of couples with Uc decreased, especially that ofUc patients. Bacterial composition, such as Firmicutes, was altered between Uc patients andhealthy controls, but was not significantly different between Uc patients and their spouses. thishas also been observed in pathways, such as metabolism, genetic information processing,organismal systems, and human diseases. however, the genes and enzymes of spouses with Ucwere not significantly different from those of healthy individuals. Furthermore, the presence ofFaecalibacterium correlated with oxidative phosphorylation, starch and sucrose metabolism, aminosugar and nucleotide sugar metabolism, and the bacterial secretion system, showed a markeddecline in the Uc group compared with their spouses, but did not vary between healthy couples.Conclusion: Our study revealed that cohabitation with Uc patients decreased differences in thegut microbiome between healthy individuals and patients. Not only was the composition anddiversity of the microbiota diminished, but active pathways also showed some decline.Furthermore, Firmicutes, Faecalibacterium, and the four related pathways may be associated with the pathological state of the host rather than with human behaviour
Experiment 1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Colon , Colorectal mucosa Hindgut,Large bowel,Posterior intestine,Colon,colon,Colorectal mucosa,colorectal mucosa
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Ulcerative colitis colitis ulcerative,Colitis, Ulcerative,Left-sided ulcerative (chronic) colitis,Left-sided ulcerative colitis,left-sided ulcerative colitis,Other ulcerative colitis,Other ulcerative colitis (disorder),UC - ulcerative colitis,ulcerative colitis,ulcerative colitis (disease),ulcerative colitis (disorder),Ulcerative colitis, unspecified,ULCERATVE COLITIS UNSPCF,Ulcerative colitis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy controls, spouses
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- ulcerative colitis patients and spouses
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- spouse living with ulcerative colitis patient
- Group 0 sample size Number of subjects in the control (unexposed) group
- 26
- Group 1 sample size Number of subjects in the case (exposed) group
- 26
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- antibiotic exclusion for three months before the start of the study
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- ANOVA
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3
- Matched on Factors on which subjects have been matched on in a case-control study
- age, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
- Richness Number of species
- decreased
