Altered gut microbiota in Parkinson's disease patients with motor complications

Study information

Reviewed Marked as Reviewed by Peace Sandy on 2024-4-5

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

34954497

DOI Digital object identifier for electronic documents.

10.1016/j.parkreldis.2021.12.012

URI

https://doi.org/10.1016/j.parkreldis.2021.12.012

Authors

Takahashi K, Nishiwaki H, Ito M, Iwaoka K, Takahashi K, Suzuki Y, Taguchi K, Yamahara K, Tsuboi Y, Kashihara K, Hirayama M, Ohno K, Maeda T

Journal

Parkinsonism & related disorders

Year

2022

Keywords:

Dyskinesia, Gut microbiota, Motor complications, Parkinson's disease, Wearing-off

INTRODUCTION: Parkinson's disease (PD) is associated with gut dysbiosis. However, whether gut dysbiosis can cause motor complications is unclear. METHODS: Subjects were enrolled from four independent movement disorder centers in Japan. We performed 16S ribosomal RNA gene sequence analysis of gut microbiota. Relative abundance of gut microbiota and relationships between them and clinical characteristics were statistically analyzed. Analysis of co-variance (ANCOVA) was used to assess altered gut microbiota associated with wearing-off or dyskinesia. RESULTS: We enrolled 223 patients with PD. Wearing-off was noted in 47.5% of patients and dyskinesia in 21.9%. We detected 98 genera of bacteria. Some changes in the gut microbiota were observed in patients with PD and motor complications. After Bonferroni correction, patients with wearing-off showed decreased relative abundance of Lachnospiraceae Blautia (p < 0.0001) and increased relative abundance of Lactobacillaceae Lactobacillus (p < 0.0001), but patients with dyskinesia no longer showed significant changes in the gut microbiota. Adjustment with two models of confounding factors followed by ANCOVA revealed that age (p < 0.0001), disease duration (p = 0.01), and wearing-off (p = 0.0004) were independent risks for the decreased relative abundance of Lachnospiraceae Blautia, and wearing-off (p = 0.009) was the only independent risk factor for the increased relative abundance of Lachnospiraceae Lactobacillus. CONCLUSION: Relative abundance of Lachnospiraceae Blautia and Lactobacillaceae Lactobacillus was significantly decreased and increased, respectively, in the gut microbiota of PD patients with motor complications. This indicates that an altered gut microbiota is associated with the development of motor complications in patients with advanced PD.

Experiment 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-4-5

Curated date: 2024/03/18

Curator: PraiseAgbetuyi

Revision editor(s): PraiseAgbetuyi, Peace Sandy

Subjects

Location of subjects: Japan

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Parkinson's disease IDIOPATHIC PARKINSON DIS,Idiopathic Parkinson Disease,Idiopathic Parkinson's Disease,IDIOPATHIC PARKINSONS DIS,Idiopathic PD,LEWY BODY PARKINSON DIS,Lewy Body Parkinson Disease,Lewy Body Parkinson's Disease,Paralysis agitans,paralysis agitans,PARKINSON DIS,PARKINSON DIS IDIOPATHIC,Parkinson disease,Parkinson Disease, Idiopathic,Parkinson syndrome,Parkinson's,Parkinson's disease,Parkinson's disease (disorder),Parkinson's disease NOS,Parkinson's disease NOS (disorder),Parkinson's Disease, Idiopathic,Parkinson's Disease, Lewy Body,Parkinson's syndrome,Parkinsonian disorder,Parkinsonism, Primary,Parkinsons,PARKINSONS DIS,PARKINSONS DIS IDIOPATHIC,PARKINSONS DIS LEWY BODY,Parkinsons disease,Primary Parkinsonism,parkinson's disease

Group 0 name Corresponds to the control (unexposed) group for case-control studies: PD patients with dyskinesia

Group 1 name Corresponds to the case (exposed) group for case-control studies: PD patients with wearing-off

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Parkinson's disease patients with wearing-off

Group 0 sample size Number of subjects in the control (unexposed) group: 106

Group 1 sample size Number of subjects in the case (exposed) group: 49

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): Within a month before the start of this study

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

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Source: Figure 1

Description: Relationships between Lachnospiraceae Blautia or Lactobacillaceae Lactobacillus and motor complications In patients with wearing-off, the relative abundance of Lachnospiraceae Blautia (A) and Lactobacillaceae Lactobacillus (C) significantly decreased and increased, respectively. Patients with dyskinesia (B and D) showed no change in relative abundance.

Abundance in Group 1: increased abundance in PD patients with wearing-off

NCBI	Quality Control	Links
Lactobacillus

Revision editor(s): PraiseAgbetuyi, Peace Sandy

Signature 2

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Reviewed Marked as Reviewed by Peace Sandy on 2024-4-5

Curated date: 2024/03/18

Curator: PraiseAgbetuyi

Revision editor(s): PraiseAgbetuyi, Peace Sandy

Source: Figure 1

Description: . Relationships between Lachnospiraceae Blautia or Lactobacillaceae Lactobacillus and motor complications In patients with wearing-off, the relative abundance of Lachnospiraceae Blautia (A) and Lactobacillaceae Lactobacillus (C) significantly decreased and increased, respectively. Patients with dyskinesia (B and D) showed no change in relative abundance.

Abundance in Group 1: decreased abundance in PD patients with wearing-off

NCBI	Quality Control	Links
Blautia

Revision editor(s): PraiseAgbetuyi, Peace Sandy