External light-dark cycle shapes gut microbiota through intrinsically photosensitive retinal ganglion cells/Experiment 6
From BugSigDB
Needs review
Subjects
- Location of subjects
- Taiwan
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Light Light,light
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Dim light at night (dLAN) - Control group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Dim light at night (dLAN) - MKO group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Exposure to aberrant light conditions such as dim light at night (dLAN) in the group with knockout of the photopigment melanopsin (MKO).
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4-V5
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2.0
- Matched on Factors on which subjects have been matched on in a case-control study
- age
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
Signature 1
Needs review
Source: Figure 4B and 4C
Description: Figure 4B and 4C represents a cladogram that shows the difference in the relative abundance of microbes from control mice and MKO mice housed under conditions of Normal light‐dark cycle (LD) and Dim light at night (dLAN).
Abundance in Group 1: decreased abundance in Dim light at night (dLAN) - MKO group
| NCBI | Quality Control | Links |
|---|---|---|
| Muribaculaceae | ||
| Paraprevotella | ||
| Lactobacillus | ||
| Lactobacillaceae | ||
| Enterococcaceae bacterium RF39 |
Revision editor(s): Aleru Divine
