The Effect of Oral Iron Supplementation on Gut Microbial Composition: a Secondary Analysis of a Double-Blind, Randomized Controlled Trial among Cambodian Women of Reproductive Age/Experiment 1
From BugSigDB
Subjects
- Location of subjects
- Cambodia
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Environmental exposure measurement Environmental exposure measurement,environmental exposure measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Placebo group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Ferrous bisglycinate group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Healthy, non pregnant women of reproductive age who received oral supplementation of ferrous bisglycinate capsule containing 18 mg of elemental iron.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 47
- Group 1 sample size Number of subjects in the case (exposed) group
- 46
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Women who were taking antibiotics in the previous 12 weeks were excluded.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V6-V8
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Beta Binomial Regression
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- Confounders controlled for: "inflammation" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.inflammation
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Figure 4, Figure 5
Description: Differentially abundant bacterial Taxa in the Ferrous bisglycinate group compared to the Placebo group.
Abundance in Group 1: increased abundance in Ferrous bisglycinate group
| NCBI | Quality Control | Links |
|---|---|---|
| Enterobacteriaceae | ||
| Escherichia/Shigella sp. | ||
| Weissella |
Revision editor(s): Manisha28
Signature 2
Source: Figure 4
Description: Differentially abundant bacterial Taxa in the Ferrous bisglycinate group compared to the Placebo group.
Abundance in Group 1: decreased abundance in Ferrous bisglycinate group
| NCBI | Quality Control | Links |
|---|---|---|
| Enterococcus |
Revision editor(s): Manisha28
