The Effect of Oral Iron Supplementation on Gut Microbial Composition: a Secondary Analysis of a Double-Blind, Randomized Controlled Trial among Cambodian Women of Reproductive Age/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2025-1-20
Subjects
- Location of subjects
- Cambodia
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Iron 26Fe,[Fe],Eisen,fer,ferrum,hierro,InChI=1/Fe,InChIKey=XEEYBQQBJWHFJM-UHFFFAOYAG,iron atom,Iron,iron
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Placebo group - 0 week treatment (Baseline)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Placebo group - 12 weeks treatment
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Placebo group - 12 weeks treatment refers to patients in the placebo group who are receiving 12 weeks treatment.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 47
- Group 1 sample size Number of subjects in the case (exposed) group
- 47
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 12 weeks
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V6-V8
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Beta Binomial Regression
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- unchanged
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-1-20
Source: Figure 4, Figure 5
Description: Differentially abundant bacterial Taxa in the Placebo group.
Abundance in Group 1: increased abundance in Placebo group - 12 weeks treatment
| NCBI | Quality Control | Links |
|---|---|---|
| Enterobacteriaceae | ||
| Enterococcus | ||
| Escherichia/Shigella sp. |
Revision editor(s): Manisha28, KateRasheed
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-1-20
Source: Figure 4
Description: Differentially abundant bacterial Taxa in the Placebo group.
Abundance in Group 1: decreased abundance in Placebo group - 12 weeks treatment
| NCBI | Quality Control | Links |
|---|---|---|
| Weissella |
Revision editor(s): Manisha28, KateRasheed
