Major urinary protein (Mup) gene family deletion drives sex-specific alterations on the house mouse gut microbiota

From BugSigDB
study design
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Real MVF, Colvin MS, Sheehan MJ, Moeller AH
bioRxiv : the preprint server for biology
The gut microbiota is shaped by host metabolism. In house mice (Mus musculus), major urinary protein (MUP) pheromone production represents a considerable energy investment, particularly in sexually mature males. Deletion of the Mup gene family shifts mouse metabolism towards an anabolic state, marked by lipogenesis, lipid accumulation, and body mass increases. Given the metabolic implications of MUPs, they may also influence the gut microbiota. Here, we investigated the effect of deletion of the Mup gene family on the gut microbiota of sexually mature mice. Shotgun metagenomics revealed distinct taxonomic and functional profiles between wildtype and knockout males, but not females. Deletion of the Mup gene cluster significantly reduced diversity in microbial families and functions in male mice. Additionally, specific taxa of the Ruminococcaceae family, which is associated with gut health and reduced risk of developing metabolic syndrome, and several microbial functions, such as transporters involved in vitamin B5 acquisition, were significantly depleted in the microbiota of Mup-knockout males. Altogether these results show that major urinary proteins significantly affect the gut microbiota of house mouse in a sex-specific manner.

Experiment 1

Needs review

Curated date: 2024/03/19

Curator: Fetlework

Revision editor(s): Fetlework


Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Hindgut , Midgut , Foregut Metenteron,Hindgut,hindgut,Mesenteron,Midgut,midgut,Praeenteron,Proenteron,Foregut,foregut
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Gene deletion library gene knockdown library,Gene repression library,Gene silencing library,Gene deletion library,gene deletion library
Group 0 name Corresponds to the control (unexposed) group for case-control studies
male mice
Group 1 name Corresponds to the case (exposed) group for case-control studies
female mice
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
female mice, including knock out and wild
Group 0 sample size Number of subjects in the control (unexposed) group
Group 1 sample size Number of subjects in the case (exposed) group

Lab analysis

Sequencing type
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Mixed-Effects Regression
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Alpha Diversity

Pielou Quantifies how equal the community is numerically
Shannon Estimator of species richness and species evenness: more weight on species richness
Richness Number of species