Characterization of Supragingival Plaque and Oral Swab Microbiomes in Children With Severe Early Childhood Caries

From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-6-5
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
de Jesus VC, Khan MW, Mittermuller BA, Duan K, Hu P, Schroth RJ, Chelikani P
Journal
Frontiers in microbiology
Year
2021
Keywords:
artificial intelligence, bacteria, case-control, dental plaque, fungi, machine learning, microbiota, oral swab
The human oral cavity harbors one of the most diverse microbial communities with different oral microenvironments allowing the colonization of unique microbial species. This study aimed to determine which of two commonly used sampling sites (dental plaque vs. oral swab) would provide a better prediction model for caries-free vs. severe early childhood caries (S-ECC) using next generation sequencing and machine learning (ML). In this cross-sectional study, a total of 80 children (40 S-ECC and 40 caries-free) < 72 months of age were recruited. Supragingival plaque and oral swab samples were used for the amplicon sequencing of the V4-16S rRNA and ITS1 rRNA genes. The results showed significant differences in alpha and beta diversity between dental plaque and oral swab bacterial and fungal microbiomes. Differential abundance analyses showed that, among others, the cariogenic species Streptococcus mutans was enriched in the dental plaque, compared to oral swabs, of children with S-ECC. The fungal species Candida dubliniensis and C. tropicalis were more abundant in the oral swab samples of children with S-ECC compared to caries-free controls. They were also among the top 20 most important features for the classification of S-ECC vs. caries-free in oral swabs and for the classification of dental plaque vs. oral swab in the S-ECC group. ML approaches revealed the possibility of classifying samples according to both caries status and sampling sites. The tested site of sample collection did not change the predictability of the disease. However, the species considered to be important for the classification of disease in each sampling site were slightly different. Being able to determine the origin of the samples could be very useful during the design of oral microbiome studies. This study provides important insights into the differences between the dental plaque and oral swab bacteriome and mycobiome of children with S-ECC and those caries-free.

Experiment 1


Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/04/21

Curator: Rahila

Revision editor(s): Rahila, Scholastica

Subjects

Location of subjects
Canada
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Supragingival dental plaque , Saliva Supragingival plaque,Supragingival dental plaque,supragingival dental plaque,Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Microbiome measurement Microbiome measurement,microbiome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Oral swab from severe early childhood caries (S-ECC)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Supragingival plaque from severe early childhood caries (S-ECC)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Supragingival plaque samples of children < 72 months of age with severe early childhood caries (S-ECC)
Group 0 sample size Number of subjects in the control (unexposed) group
40
Group 1 sample size Number of subjects in the case (exposed) group
40
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
Use of antibiotics

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Chao1 Abundance-based estimator of species richness
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/04/21

Curator: Rahila

Revision editor(s): Rahila, Scholastica

Source: FIG. 3B

Description: Differential abundance analysis of species that were overabundant in supragingival plaque compared to oral swab samples within the S-ECC (severe early childhood caries) group

Abundance in Group 1: increased abundance in Supragingival plaque from severe early childhood caries (S-ECC)

NCBI Quality ControlLinks
Abiotrophia defectiva
Capnocytophaga sputigena
Corynebacterium durum
Corynebacterium matruchotii
Kingella kingae
Kingella oralis
Lautropia mirabilis
Rothia aeria
Rothia dentocariosa
Segatella maculosa
Segatella oris
Selenomonas artemidis
Selenomonas sputigena
Streptococcus anginosus
Streptococcus mutans
Streptococcus salivarius
Prevotella sp. HMT 472Prevotella sp. HMT 472
Actinomyces sp. HMT 180Actinomyces sp. HMT 180
Leptotrichia sp. HMT 498Leptotrichia sp. HMT 498
Ottowia sp. HMT 894Ottowia sp. HMT 894
Kingella sp. HMT 012Kingella sp. HMT 012
Capnocytophaga sp. HMT 326Capnocytophaga sp. HMT 326
Mitsuokella sp. HMT 131Mitsuokella sp. HMT 131

Revision editor(s): Rahila, Scholastica

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/04/21

Curator: Rahila

Revision editor(s): Rahila, Scholastica

Source: FIG. 3B

Description: Differential abundance analysis of species that were overabundant in supragingival plaque compared to oral swab samples within the S-ECC (severe early childhood caries) group

Abundance in Group 1: decreased abundance in Supragingival plaque from severe early childhood caries (S-ECC)

NCBI Quality ControlLinks
Gemella morbillorum
Granulicatella elegans
Haemophilus influenzae
Haemophilus parainfluenzae
Hoylesella nanceiensis
Simonsiella muelleri
Sneathia vaginalis
Porphyromonas sp. HMT 930Porphyromonas sp. HMT 930
Bergeyella sp. HMT 931Bergeyella sp. HMT 931
Alloprevotella sp. HMT 473Alloprevotella sp. HMT 473
Veillonella sp. HMT 780Veillonella sp. HMT 780

Revision editor(s): Rahila, Scholastica

Experiment 2


Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/04/21

Curator: Rahila

Revision editor(s): Rahila, Scholastica

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Saliva , Supragingival dental plaque Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva,Supragingival plaque,Supragingival dental plaque,supragingival dental plaque
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Oral swab from caries-free
Group 1 name Corresponds to the case (exposed) group for case-control studies
Supragingival plaque from caries-free
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Supragingival plaque samples of children < 72 months of age who are caries-free, confirmed by a dental examination

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Chao1 Abundance-based estimator of species richness
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/04/21

Curator: Rahila

Revision editor(s): Rahila, Scholastica

Source: FIG. 3C

Description: Differential abundance analysis of species that were overabundant in supragingival plaque compared to oral swab samples within the caries-free group

Abundance in Group 1: increased abundance in Supragingival plaque from caries-free

NCBI Quality ControlLinks
Abiotrophia defectiva
Actinomyces gerencseriae
Aggregatibacter aphrophilus
Campylobacter gracilis
Campylobacter rectus
Capnocytophaga gingivalis
Capnocytophaga granulosa
Capnocytophaga leadbetteri
Capnocytophaga sputigena
Cardiobacterium hominis
Corynebacterium durum
Corynebacterium matruchotii
Eikenella corrodens
Kingella oralis
Lautropia mirabilis
Leptotrichia hongkongensis
Rothia aeria
Rothia dentocariosa
Selenomonas artemidis
Selenomonas sputigena
Streptococcus intermedius
Bergeyella sp. HMT 322Bergeyella sp. HMT 322
Fusobacterium sp. HMT 203Fusobacterium sp. HMT 203
P.[XI][G-7] yurii subspp. yurii & margaretiaeP.[XI][G-7] yurii subspp. yurii & margaretiae
Prevotella sp. HMT 472Prevotella sp. HMT 472
Prevotella sp. HMT 317Prevotella sp. HMT 317
Lachnospiraceae [G-3] bacterium HMT 100Lachnospiraceae [G-3] bacterium HMT 100
Capnocytophaga sp. HMT 326Capnocytophaga sp. HMT 326
Leptotrichia sp. HMT 392Leptotrichia sp. HMT 392
Leptotrichia sp. HMT 212Leptotrichia sp. HMT 212
Absconditabacteria (SR1) [G-1] bacterium HMT 345Absconditabacteria (SR1) [G-1] bacterium HMT 345
Kingella sp. HMT 012Kingella sp. HMT 012

Revision editor(s): Rahila, Scholastica

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/04/21

Curator: Rahila

Revision editor(s): Rahila, Scholastica

Source: FIG. 3C

Description: Differential abundance analysis of species that were overabundant in supragingival plaque compared to oral swab samples within the caries-free group

Abundance in Group 1: decreased abundance in Supragingival plaque from caries-free

NCBI Quality ControlLinks
Gemella morbillorum
Granulicatella elegans
Haemophilus parainfluenzae
Porphyromonas pasteri
Prevotella melaninogenica
Rothia mucilaginosa
Sneathia vaginalis
Porphyromonas sp. HMT 930Porphyromonas sp. HMT 930
Veillonella sp. HMT 780Veillonella sp. HMT 780
Alloprevotella sp. HMT 473Alloprevotella sp. HMT 473

Revision editor(s): Rahila, Scholastica

Experiment 3


Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/06/05

Curator: Scholastica

Revision editor(s): Scholastica

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Supragingival dental plaque , Saliva Supragingival plaque,Supragingival dental plaque,supragingival dental plaque,Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Oral swab from severe early childhood caries (S-ECC)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Supragingival plaque from severe early childhood caries (S-ECC)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Supragingival plaque samples of children < 72 months of age with severe early childhood caries (S-ECC)

Lab analysis

Sequencing type
ITS / ITS2
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/06/05

Curator: Scholastica

Revision editor(s): Scholastica

Source: Table 2

Description: Differential abundance analysis of fungal taxa in supragingival plaque compared to oral swab samples within the S-ECC (severe early childhood caries) group

Abundance in Group 1: increased abundance in Supragingival plaque from severe early childhood caries (S-ECC)

NCBI Quality ControlLinks
Candida dubliniensis
Candida albicans
Thelephoraceae
Ramicandelaber taiwanensis
Fusarium sp.
Stereum rugosum
Wallemia tropicalis

Revision editor(s): Scholastica

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-6-6

Curated date: 2024/06/05

Curator: Scholastica

Revision editor(s): Scholastica

Source: Table 2

Description: Differential abundance analysis of fungal taxa in supragingival plaque compared to oral swab samples within the S-ECC (severe early childhood caries) group

Abundance in Group 1: decreased abundance in Supragingival plaque from severe early childhood caries (S-ECC)

NCBI Quality ControlLinks
Agaricomycetes
Malassezia restricta
Trichosporon asahii
Malassezia globosa

Revision editor(s): Scholastica

Experiment 4


Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/06/05

Curator: Scholastica

Revision editor(s): Scholastica

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Saliva , Supragingival dental plaque Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva,Supragingival plaque,Supragingival dental plaque,supragingival dental plaque
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Oral swab from caries-free
Group 1 name Corresponds to the case (exposed) group for case-control studies
Supragingival plaque from caries-free
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Supragingival plaque samples of children < 72 months of age who are caries-free, confirmed by a dental examination

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
decreased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/06/05

Curator: Scholastica

Revision editor(s): Scholastica

Source: Table 2

Description: Differential abundance analysis of fungal taxa in supragingival plaque compared to oral swab samples within the caries-free group

Abundance in Group 1: increased abundance in Supragingival plaque from caries-free

NCBI Quality ControlLinks
Blumeria sp.
Malassezia restricta
Trichosporon asahii
Ramicandelaber taiwanensis
Fusarium sp.
Meyerozyma guilliermondii
Malasseziales
Cryptomycota
Chytridiomycota
Wallemia tropicalis

Revision editor(s): Scholastica

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/06/05

Curator: Scholastica

Revision editor(s): Scholastica

Source: Table 2

Description: Differential abundance analysis of fungal taxa in supragingival plaque compared to oral swab samples within the caries-free group

Abundance in Group 1: decreased abundance in Supragingival plaque from caries-free

NCBI Quality ControlLinks
Agaricomycetes
Ascomycota

Revision editor(s): Scholastica

Experiment 5


Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/06/05

Curator: Scholastica

Revision editor(s): Scholastica

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
Group 1 name Corresponds to the case (exposed) group for case-control studies
Oral swab from severe early childhood caries (S-ECC)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Oral swap samples of children < 72 months of age who are caries-free, confirmed by a dental examination

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/06/05

Curator: Scholastica

Revision editor(s): Scholastica

Source: Table 2

Description: Differential abundance analysis of fungal taxa in oral swab samples within the caries-free group versus oral swab samples within the S-ECC (severe early childhood caries) group

Abundance in Group 1: increased abundance in Oral swab from severe early childhood caries (S-ECC)

NCBI Quality ControlLinks
Candida dubliniensis
Malassezia restricta
Candida tropicalis

Revision editor(s): Scholastica

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/06/05

Curator: Scholastica

Revision editor(s): Scholastica

Source: Table 2

Description: Differential abundance analysis of fungal taxa in oral swab samples within the caries-free group versus oral swab samples within the S-ECC (severe early childhood caries) group

Abundance in Group 1: decreased abundance in Oral swab from severe early childhood caries (S-ECC)

NCBI Quality ControlLinks
Thelephoraceae

Revision editor(s): Scholastica