Current levels of microplastic pollution impact wild seabird gut microbiomes/Experiment 1

From BugSigDB


Needs review

Curated date: 2024/04/20

Curator: Bolanle

Revision editor(s): Bolanle

Subjects

Location of subjects
Portugal
Host species Species from which microbiome was sampled. Contact us to have more species added.
Calonectris borealis
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Proventriculus , Cloaca Proventriculus,proventriculus,Cloacal chamber,Vent,Cloaca,cloaca
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Exposure Exposure,exposure
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Proventricular microbiome in Cory's sheawater individuals
Group 1 name Corresponds to the case (exposed) group for case-control studies
Cloacal microbiome in Cory's sheawater individuals
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
effect of microplastic count in the Cloacal microbiome in Cory's sheawater individuals
Group 0 sample size Number of subjects in the control (unexposed) group
85
Group 1 sample size Number of subjects in the case (exposed) group
84

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
centered log-ratio
Statistical test
ANCOM
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Richness Number of species
increased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
increased

Signature 1

Needs review

Curated date: 2024/04/30

Curator: Bolanle

Revision editor(s): Bolanle

Source: figure 2

Description: Differential abundance of microplastic counts

Abundance in Group 1: increased abundance in Cloacal microbiome in Cory's sheawater individuals

NCBI Quality ControlLinks
Cetobacterium

Revision editor(s): Bolanle

Signature 2

Needs review

Curated date: 2024/04/30

Curator: Bolanle

Revision editor(s): Bolanle

Source: figure 2

Description: Differential abundance of microplastic counts

Abundance in Group 1: decreased abundance in Cloacal microbiome in Cory's sheawater individuals

NCBI Quality ControlLinks
Fusobacterium sp.
Edwardsiella
Pseudoalteromonas sp.
catellicoccuscatellicoccus

Revision editor(s): Bolanle