Sex, health status and habitat alter the community composition and assembly processes of symbiotic bacteria in captive frogs/Experiment 1
From BugSigDB
Needs review
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Pelophylax nigromaculatus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Intestine Bowel,Intestinal tract,Intestine,intestine
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Sex design sex_design,Sex design,sex design
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- male
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- female
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- female black spotted frog
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Needs review
Source: figure 3a
Description: differential abundance of bacteria genera in frog guts between female and male frog samples.
Abundance in Group 1: increased abundance in female
| NCBI | Quality Control | Links |
|---|---|---|
| Oscillospiraceae |
Revision editor(s): Idiaru angela
Signature 2
Needs review
Source: figure 3a
Description: Differential abundance of bacterial genera in frog guts between male and female frog samples.
Abundance in Group 1: decreased abundance in female
| NCBI | Quality Control | Links |
|---|---|---|
| Laribacter |
Revision editor(s): Idiaru angela
