A cross-sectional analysis about bacterial vaginosis, high-risk human papillomavirus infection, and cervical intraepithelial neoplasia in Chinese women
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Xu X, Zhang Y, Yu L, Shi X, Min M, Xiong L, Pan J, Liu P, Wu G, Gao G
Journal
Scientific reports
Year
2022
Bacterial vaginosis (BV) is a genital infection that frequently presents in women infected with human papillomavirus (HPV), but the correlation between BV, HPV and cervical intraepithelial neoplasia (CIN) development is still elusive. We organized a cross-sectional analysis which enrolled 624 participants and obtained 423 samples of vaginal secretions from them, including 193 HPV-negative samples and 230 HR-HPV-positive samples. We used 16S rRNA sequencing to measure the vaginal microbiota diversity in women with different BV, HPV and CIN status, and then calculated risk factors for CIN by logistic regression. We found that the diversity of vaginal microbiota was significantly increased after BV, HPV and BV-infected CIN group. The Observed species and Chao1 index of H.C group showed little difference with normal group, while its Shannon index was considerable higher than normal group. L. iners enriched in HPV infection group compared with others significantly. BV (OR = 0.358; 95% CI = 0.195-0.656; P < .05) and HR-HPV infection (OR = 0.016; 95% CI = 0.004-0.072; P < .001) were risk factors for CIN. In conclusion, we consider BV as a risk factor for CIN. The enrichment of L. iners under HPV infection state may contribute to maintenance of vaginal dysbiosis, and BV infection could facilitate the disturb.
Experiment 1
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Uterine cervix , Vaginal fluid Canalis cervicis uteri,Caudal segment of uterus,Cervical canal,Cervical canal of uterus,Cervix,Cervix of uterus,Cervix uteri,Neck of uterus,Uterine cervix,uterine cervix,Vaginal discharge,Vaginal secretion,Vaginal fluid,vaginal fluid
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Vaginal microbiome measurement vaginal flora measurement,vaginal microbiota measurement,Vaginal microbiome measurement,vaginal microbiome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal Healthy Women & High‑risk human papillomavirus(HR-HPV) Infected Women (HPV)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Bacterial Vaginosis (BV) Infected Women
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Women attending the Aviation General hospital, located in Beijing, China, who were diagnosed with Bacterial Vaginosis (BV) Infection.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 136
- Group 1 sample size Number of subjects in the case (exposed) group
- 52
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 30 days
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 4
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Source: Figure 5A
Description: Bacterial Taxa differentially abundant in the vaginal microbiome of Bacterial Vaginosis (BV) Infected women compared to Normal healthy women & High‑risk human papillomavirus(HR-HPV) Infected Women as determined by LEfSe Analysis.
Abundance in Group 1: increased abundance in Bacterial Vaginosis (BV) Infected Women
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidales | ||
| Bacteroidia | ||
| Bacteroidota | ||
| Hoylesella timonensis | ||
| Prevotella | ||
| Prevotellaceae |
Revision editor(s): Joan Chuks
Experiment 2
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal Healthy Women & Bacterial Vaginosis (BV) Infected Women
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- High‑risk human papillomavirus(HR-HPV) Infected Women (HPV)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Women attending the Aviation General hospital, located in Beijing, China, who were diagnosed with High‑risk human papillomavirus (HR-HPV) infection.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 117
- Group 1 sample size Number of subjects in the case (exposed) group
- 71
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Source: Figure 5A
Description: Bacterial Taxa differentially abundant in the vaginal microbiome of High‑risk human papillomavirus(HR-HPV) Infected Women compared to Normal healthy women & Bacterial Vaginosis (BV) Infected Women as determined by LEfSe Analysis.
Abundance in Group 1: increased abundance in High‑risk human papillomavirus(HR-HPV) Infected Women (HPV)
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillus iners |
Revision editor(s): Joan Chuks
Experiment 3
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal Healthy Women
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Bacterial vaginosis (BV) infected women & High‑risk human papillomavirus (HR-HPV) (HPV) infected Women
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Women attending the Aviation General hospital, located in Beijing, China, who were diagnosed with Bacterial vaginosis (BV) infection and those diagnosed with High‑risk human papillomavirus (HR-HPV) infection.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 65
- Group 1 sample size Number of subjects in the case (exposed) group
- 123
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Source: Figure 5A
Description: Bacterial Taxa differentially abundant in the vaginal microbiome of Bacterial Vaginosis (BV) infected women and High‑risk human papillomavirus (HR-HPV) infected women compared to Normal healthy women as determined by LEfSe Analysis.
Abundance in Group 1: decreased abundance in Bacterial vaginosis (BV) infected women & High‑risk human papillomavirus (HR-HPV) (HPV) infected Women
| NCBI | Quality Control | Links |
|---|---|---|
| Bacilli | ||
| Bacillota | ||
| Lactobacillaceae | ||
| Lactobacillales | ||
| Lactobacillus |
Revision editor(s): Joan Chuks
Experiment 4
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- High‑risk human papillomavirus(HR-HPV) & Cervical Intraepithelial Neoplasia(CIN) co-infected women (HC)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Women attending the Aviation General hospital, located in Beijing, China, who were diagnosed with infection of High‑risk human papillomavirus (HR-HPV) combined with Cervical Intraepithelial Neoplasia (CIN).
- Group 1 sample size Number of subjects in the case (exposed) group
- 48
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
Experiment 5
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Bacteial Vaginosis (BV) & High‑risk human papillomavirus(HR-HPV) with Cervical Intraepithelial Neoplasia(CIN) co-infected women (BHC)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Women attending the Aviation General hospital, located in Beijing, China, who were diagnosed with co-infection of Bacteial Vaginosis (BV) & High‑risk human papillomavirus (HR-HPV) in combination with Cervical Intraepithelial Neoplasia (CIN).
- Group 1 sample size Number of subjects in the case (exposed) group
- 46
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
Experiment 6
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Bacterial Vaginosis (BV) Infected Women
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- High‑risk human papillomavirus(HR-HPV) & Cervical Intraepithelial Neoplasia(CIN) co-infected women (HC)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Women attending the Aviation General hospital, located in Beijing, China, who were diagnosed with infection of High‑risk human papillomavirus (HR-HPV) combined with Cervical Intraepithelial Neoplasia (CIN).
- Group 0 sample size Number of subjects in the control (unexposed) group
- 52
- Group 1 sample size Number of subjects in the case (exposed) group
- 48
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
Experiment 7
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- High‑risk human papillomavirus (HR-HPV) Infected Women (HPV)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 71
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
Experiment 8
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Bacterial vaginosis (BV) and High‑risk human papillomavirus (HR-HPV) (HPV) co-infected Women (BH)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 60
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
Experiment 9
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- High‑risk human papillomavirus(HR-HPV) Infected Women (HPV)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Bacteial Vaginosis (BV) & High‑risk human papillomavirus(HR-HPV) with Cervical Intraepithelial Neoplasia(CIN) co-infected women (BHC)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Women attending the Aviation General hospital, located in Beijing, China, who were diagnosed with with co-infection of Bacteial Vaginosis (BV) & High‑risk human papillomavirus (HR-HPV) in combination with Cervical Intraepithelial Neoplasia (CIN).
- Group 0 sample size Number of subjects in the control (unexposed) group
- 71
- Group 1 sample size Number of subjects in the case (exposed) group
- 46
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
Experiment 10
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- High‑risk human papillomavirus(HR-HPV) & Cervical Intraepithelial Neoplasia(CIN) co-infected women (HC)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 48
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
Experiment 11
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal Healthy Women
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Bacterial Vaginosis (BV) Infected Women
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Women attending the Aviation General hospital, located in Beijing, China, who were diagnosed with Bacterial Vaginosis (BV) Infection.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 65
- Group 1 sample size Number of subjects in the case (exposed) group
- 52
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- Not specified
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
Experiment 12
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- High‑risk human papillomavirus(HR-HPV) Infected Women (HPV)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Women attending the Aviation General hospital, located in Beijing, China, who were diagnosed with High‑risk human papillomavirus (HR-HPV) infection.
- Group 1 sample size Number of subjects in the case (exposed) group
- 71
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
Experiment 13
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Bacterial vaginosis (BV) and High‑risk human papillomavirus (HR-HPV) (HPV) co-infected Women (BH)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Women attending the Aviation General hospital, located in Beijing, China, who were diagnosed with co-infection of Bacterial vaginosis (BV) and High‑risk human papillomavirus (HR-HPV).
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
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