Sex differences in gut microbiota in patients with major depressive disorder

From BugSigDB
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Chen JJ, Zheng P, Liu YY, Zhong XG, Wang HY, Guo YJ, Xie P
Journal
Neuropsychiatric disease and treatment
Year
2018
Keywords:
MDD, biomarker, gut microbiota, major depressive disorder
Objective: Our previous studies found that disturbances in gut microbiota might have a causative role in the onset of major depressive disorder (MDD). The aim of this study was to investigate whether there were sex differences in gut microbiota in patients with MDD. Patients and methods: First-episode drug-naïve MDD patients and healthy controls were included. 16S rRNA gene sequences extracted from the fecal samples of the included subjects were analyzed. Principal-coordinate analysis and partial least squares-discriminant analysis were used to assess whether there were sex-specific gut microbiota. A random forest algorithm was used to identify the differential operational taxonomic units. Linear discriminant-analysis effect size was further used to identify the dominant sex-specific phylotypes responsible for the differences between MDD patients and healthy controls. Results: In total, 57 and 74 differential operational taxonomic units responsible for separating female and male MDD patients from their healthy counterparts were identified. Compared with their healthy counterparts, increased Actinobacteria and decreased Bacteroidetes levels were found in female and male MDD patients, respectively. The most differentially abundant bacterial taxa in female and male MDD patients belonged to phyla Actinobacteria and Bacteroidia, respectively. Meanwhile, female and male MDD patients had different dominant phylotypes. Conclusion: These results demonstrated that there were sex differences in gut microbiota in patients with MDD. The suitability of Actinobacteria and Bacteroidia as the sex-specific biomarkers for diagnosing MDD should be further explored.

Experiment 1


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Unipolar depression Depression,Depression, Emotional,Depression, Endogenous,Depression, Involutional,Depression, Neurotic,Depression, Unipolar,Depressions,Depressions, Emotional,Depressions, Endogenous,Depressions, Neurotic,Depressions, Unipolar,DEPRESSIVE DIS,DEPRESSIVE DIS MAJOR,Depressive Disorder,Depressive Disorder, Major,Depressive Disorders,Depressive Disorders, Major,Depressive Neuroses,Depressive Neurosis,Depressive Symptom,Depressive Symptoms,Depressive Syndrome,Depressive Syndromes,Disorder, Depressive,Disorder, Major Depressive,Disorders, Depressive,Disorders, Major Depressive,Emotional Depression,Emotional Depressions,Endogenous Depression,Endogenous Depressions,Involutional Depression,Involutional Psychoses,Involutional Psychosis,MAJOR DEPRESSIVE DIS,major depressive disorder,Major Depressive Disorders,Neuroses, Depressive,Neurosis, Depressive,Neurotic Depression,Neurotic Depressions,Paraphrenia, Involutional,Psychoses, Involutional,Psychosis, Involutional,Symptom, Depressive,Symptoms, Depressive,Syndrome, Depressive,Syndromes, Depressive,Unipolar Depressions,Unipolar depression,unipolar depression
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy Controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
depressed female
Group 0 sample size Number of subjects in the control (unexposed) group
24
Group 1 sample size Number of subjects in the case (exposed) group
24
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
yes

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V5
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
Matched on Factors on which subjects have been matched on in a case-control study
demographics


Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Figure 5

Description: Sex differences in gut microbiota in female patients with major depressive disorder

Abundance in Group 1: increased abundance in depressed female

NCBI Quality ControlLinks
Blautia
Actinomycetota
Actinomyces
Bifidobacterium
Asaccharobacter
Atopobium
Eggerthella
Gordonibacter
Olsenella
Eubacterium
Anaerostipes
Roseburia
Faecalibacterium
Desulfovibrio
Coriobacteriales
Lachnospiraceae
Bifidobacteriaceae
Bifidobacteriales
Pasteurellales
Pasteurellaceae
Eubacteriaceae
Coriobacteriaceae

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Figure 5

Description: Sex differences in gut microbiota in female patients with major depressive disorder

Abundance in Group 1: decreased abundance in depressed female

NCBI Quality ControlLinks
Howardella
Sutterella
Pyramidobacter

Revision editor(s): WikiWorks

Experiment 2


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
depressed male
Group 0 sample size Number of subjects in the control (unexposed) group
20
Group 1 sample size Number of subjects in the case (exposed) group
20

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Fatima on 2021/08/10

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): Fatima, WikiWorks

Source: Figure 6

Description: Sex differences in gut microbiota in male patients with major depressive disorder

Abundance in Group 1: increased abundance in depressed male

NCBI Quality ControlLinks
Atopobium
Bacteroidaceae
Bacteroides
Bacteroidia
Veillonella
Erysipelotrichaceae incertae sedis

Revision editor(s): Fatima, WikiWorks

Signature 2

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Figure 6

Description: Sex differences in gut microbiota in male patients with major depressive disorder

Abundance in Group 1: decreased abundance in depressed male

NCBI Quality ControlLinks
Coriobacteriaceae
Clostridia
Gordonibacter
Anaerovorax
Pyramidobacter

Revision editor(s): WikiWorks