Gut Microbiota Composition Changes in Constipated Women of Reproductive Age

Study information

Reviewed Marked as Reviewed by Svetlana up on 2024-5-13

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

33552996

DOI Digital object identifier for electronic documents.

10.3389/fcimb.2020.557515

URI

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859112/

Authors

Li H, Chen J, Ren X, Yang C, Liu S, Bai X, Shan S, Dong X

Journal

Frontiers in cellular and infection microbiology

Year

2020

Keywords:

16S rRNA gene sequencing, chronic constipation, gut microbiota, influence factors, women of reproductive age

BACKGROUND: Chronic constipation is one of the most prevalent functional gastrointestinal disorders, yet its etiology is multifactorial, and the pathophysiological mechanism is still unclear. Previous studies have shown that the gut microbiota of constipated patients differs from healthy controls; however, many discrepancies exist in the findings, and no clear link has been confirmed between chronic constipation and changes in the gut microbiota. Growing evidence indicates that age, gender, and hormone levels can affect the composition of gut microbiota. The aim of this study is to examine the overall changes in gut microbiota within a specific sub-population of patients, namely, constipated women of reproductive age. METHODS: We carried out a cross-sectional study comparing the fecal microbial composition of 30 healthy women and 29 constipated women using 16S rRNA gene sequencing. Only women of reproductive age were recruited to reduce the effects of age, gender, and hormone levels on the microbiome, and to prevent conflating the impact of these factors with the effects of constipation. RESULTS: There were obvious differences in the gut microbiota in constipated women of reproductive age compared with the healthy controls, manifesting mainly as a significant increase in the abundance of Bacteroides (p < 0.05) and a significant decrease in the abundance of Proteobacteria (p < 0.01). The overall composition of the gut microbiota in each group was different, which was reflected in the ratios of Firmicutes to Bacteroidetes (F/B), which was 1.52 in the constipated group vs. 2.21 in the healthy group. Additionally, there was a significant decrease in butyrate-producing bacteria, like Roseburia and Fusicatenibacter (p < 0.01). CONCLUSION: The overall composition of the gut microbiota changed in constipated women of reproductive age, characterized by a loss in Proteobacteria and an increase in Bacteroidetes. Furthermore, the abundance of some butyrate-producing bacteria also reduced. These changes may reflect the unique interactions between host and some bacteria, or some bacterial metabolic products, which may be important targets for future studies to explore the pathogenesis of constipation.

Experiment 1

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Reviewed Marked as Reviewed by Svetlana up on 2024-5-13

Curated date: 2024/04/26

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Chronic constipation Chronic constipation,Infrequent bowel movements,chronic constipation

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy women

Group 1 name Corresponds to the case (exposed) group for case-control studies: Constipated women

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Female constipated patients of reproductive age.

Group 0 sample size Number of subjects in the control (unexposed) group: 30

Group 1 sample size Number of subjects in the case (exposed) group: 29

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): One month preceding sample collection.

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 4

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Svetlana up on 2024-5-13

Curated date: 2024/04/26

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table 2 and Figure3f

Description: Significant Differences in the Microbiota of Constipated Women of Reproductive Age and Healthy Controls.

Abundance in Group 1: increased abundance in Constipated women

NCBI	Quality Control	Links
Bacteroidaceae
Bacteroidales
Bacteroides
Bacteroidia
Bacteroidota
Negativicutes
Phocaeicola coprocola
Selenomonadales
Fusicatenibacter
Lactobacillaceae
Tannerellaceae
Parabacteroides
Citrobacter

Revision editor(s): Aleru Divine

Signature 2

Edit History Delete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Svetlana up on 2024-5-13

Curated date: 2024/04/26

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table 2 and Figure3f

Description: Significant Differences in the Microbiota of Constipated Women of Reproductive Age and Healthy Controls.

Abundance in Group 1: decreased abundance in Constipated women

NCBI	Quality Control	Links
Enterobacterales
Enterobacteriaceae
Escherichia coli
Gammaproteobacteria
Klebsiella
Klebsiella pneumoniae
Pseudomonadota
unclassified Enterobacteriaceae
Roseburia

Revision editor(s): Aleru Divine