Western Kenyan Anopheles gambiae showing intense permethrin resistance harbour distinct microbiota/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-6-7
Curated date: 2024/04/29
Curator: Idiaru angela
Revision editor(s): Idiaru angela, Scholastica, Svetlana up
Subjects
- Location of subjects
- Kenya
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Anopheles gambiae
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Body proper Body,Whole body,Body proper,body proper
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Insecticide insecticides,Insecticide,insecticide
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- permethrin susceptible mosquito
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- permethrin resistant mosquito
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mosquitoes that were alive after the bioassay with permethrin insecticide were categorized as resistant
- Group 0 sample size Number of subjects in the control (unexposed) group
- 36
- Group 1 sample size Number of subjects in the case (exposed) group
- 39
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-6-7
Source: Figure 5
Description: Differentially abundant bacterial genera between permethrin resistant and susceptible mosquitoes determined by Lefse analysis
Abundance in Group 1: increased abundance in permethrin resistant mosquito
Revision editor(s): Idiaru angela
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-6-7
Source: Figure 5
Description: Differentially abundant bacterial genera between permethrin resistant and susceptible mosquitoes determined by Lefse analysis
Abundance in Group 1: decreased abundance in permethrin resistant mosquito
| NCBI | Quality Control | Links |
|---|---|---|
| Myxococcales | ||
| Deltaproteobacteria | ||
| Myxococcus | ||
| Myxococcaceae |
Revision editor(s): Idiaru angela
